Annexin A4 Antibody (694624) [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Annexin A4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and the nuclear envelope of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Annexin A4 at approximately 41 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Annexin A4 Monoclonal Antibody (Catalog # MAB4146). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 694624
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Annexin A4 Antibody (694624) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Annexin A4
  Met1-Asp319
  Accession # P09525
• Specificity: Detects human Annexin A4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A5, A6, A8, A9, A10, A11, or 13A is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A4 Antibody (694624) [Unconjugated]

• AIV
• Annexin A4
• annexin IV (placental anticoagulant protein II)
• Annexin IV
• annexin-4
• ANX4
• ANX4Carbohydrate-binding protein p33/p41
• ANXA4
• Chromobindin-4
• chromobindin-4,35-beta calcimedin
• DKFZp686H02120
• Endonexin I
• Lipocortin IV
• MGC75105
• P32.5
• PAP-II
• PIG28
• Placental anticoagulant protein II
• PP4-X
• proliferation-inducing gene 28
• proliferation-inducing protein 28
• Protein II
• ZAP36

Background

Annexin A4 (ANXA4), also known as Lipocortin IV, placental anticoagulant protein II, and zymogen granule membrane-associated protein of 36 kDa (ZAP36), is a member of the Annexin protein family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Human Annexin A4 shares 92% aa sequence identity with mouse and rat Annexin A4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A4 Antibody (MAB4146)(2)

Publications using MAB4146

• A Mularski, SL Sønder, ASB Heitmann, J Nylandsted, AC Simonsen Simultaneous membrane binding of Annexin A4 and A5 suppresses 2D lattice formation while maintaining curvature induction Journal of colloid and interface science, 2021-05-18;600(0):854-864. 2021-05-18 [PMID: 34052534] (Western Blot, Human)
• TL Boye, K Maeda, W Pezeshkian, SL Sønder, SC Haeger, V Gerke, AC Simonsen, J Nylandsted Annexin A4 and A6 induce membrane curvature and constriction during cell membrane repair Nat Commun, 2017-11-20;8(1):1623. 2017-11-20 [PMID: 29158488] (ICC, Western Blot, Human)

Bioinformatics

• Gene Symbol: ANXA4
• Uniprot:
  • P09525()