Annexin A2 Antibody

Images

 
Genetic Strategies: Western Blot: Annexin A2 Antibody [NBP1-31310] - Wild-type (WT) and Annexin II knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ...read more
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody [NBP1-31310] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Annexin A2 Antibody antibody (Green) at 1:500 ...read more
Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Analysis of paraffin-embedded Human pancreas tissue, using ANXA2 antibody at 1:100 dilution.
Western Blot: Annexin A2 Antibody [NBP1-31310] - A. 30 ug A549 whole cell lysate/extract, B. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: Annexin A2 Antibody [NBP1-31310] - Analysis. Non-transfected (-) and human Annexin I or Annexin II-transfected (+) whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted ...read more
Immunohistochemistry-Paraffin: Annexin A2 Antibody [NBP1-31310] - Analysis of paraffin-embedded Human pancreas tissue, using ANXA2 antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Annexin A2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Annexin II. The exact sequence is proprietary.
Localization
Secreted, extracellular space, extracellular matrix, basement membrane, Melanosome
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Sheep (100%), Bovine (100%), Canine (100%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ANXA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Annexin A2 Lysate (NBP2-65301)
Publications
Read Publications using NBP1-31310.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Annexin A2 Antibody

  • Annexin A2
  • Annexin II
  • annexin-2
  • ANX2
  • ANX2L4
  • ANX2L4LPC2
  • ANXA2
  • CAL1H
  • Calpactin I heavy chain
  • calpactin I heavy polypeptide
  • Calpactin-1 heavy chain
  • chromobindin 8
  • chromobindin-8
  • LIP2
  • LIP2PAP-IV
  • Lipocortin II
  • Lipocortin-2
  • LPC2D
  • LPC2DP36
  • p36
  • PAP-IV
  • Placental anticoagulant protein IV
  • Protein I

Background

The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium regulated, membrane binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Annexin A2 Antibody (NBP1-31310)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Annexin A2 Antibody (NBP1-31310). (Showing 1 - 1 of 1 FAQs).

  1. I would like to know how the immunogen was produced and purified.
    • The immunogen is a synthetic peptide, so it was not raised in any expression system. The peptide is conjugated to KLH prior to immunization.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Annexin A2 Antibody (NBP1-31310)

Discover related pathways, diseases and genes to Annexin A2 Antibody (NBP1-31310). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Annexin A2 Antibody (NBP1-31310)

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Blogs on Annexin A2.

Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits....  Read full blog post.

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Bioinformatics

Gene Symbol ANXA2
Entrez
Uniprot