Annexin A13 Antibody

Western blot shows lysates of human and mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse Annexin A13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Annexin A13 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Annexin A13 Antibody Summary

• Immunogen: E. coli-derived recombinant human Annexin A13
  Met1-His316
  Accession # P27216
• Specificity: Detects endogenous human and mouse Annexin A13 in Western blots. In Western blots, this antibody did not cross-react with recombinant human Annexin A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, or A11.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANXA13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Annexin A13 Antibody

• Annexin A13
• Annexin XIII
• Annexin-13
• ANX13
• ANX13annexin, intestine-specific
• ANXA13
• Intestine-specific annexin
• ISA
• ISAannexin-13
• MGC150460

Background

Annexin A13 (ANXA13), also known as Annexin XIII and Intestine-Specific Annexin, is a member of the Annexin protein family. Two isoforms have been identified resulting from alternate splicing. Isoform A consists of 316 amino acids and has a predicted molecular weight of 36 kDa whereas isoform B is composed of 357 amino acids and has a predicted molecular weight of 40 kDa. Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin’s have a molecular weight of approximately 35 to 40 kDa and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C terminal domain is comprised of four 60-70 amino acid repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha -helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. The specific function of Annexin A13 has not been determined but it is associated with the plasma membrane of undifferentiated, proliferating endothelial cells and differentiated villus enterocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Annexin A13 Antibody (AF4149)(3)

Publications using AF4149

• Guozhong Jiang, Pengju Wang, Weiwei Wang, Wencai Li, Liping Dai, Kuisheng Chen Annexin A13 promotes tumor cell invasion in vitro and is associated with metastasis in human colorectal cancer Oncotarget 2017-03-28 [PMID: 28423508] (Western Blot, Human)
• Guozhong Jiang, Pengju Wang, Weiwei Wang, Wencai Li, Liping Dai, Kuisheng Chen Annexin A13 promotes tumor cell invasion in vitro and is associated with metastasis in human colorectal cancer Oncotarget 3/28/2017 [PMID: 28423508]
• Linke B, Abeler-Dorner L, Jahndel V, Kurz A, Mahr A, Pfrang S, Linke L, Krammer P, Weyd H The tolerogenic function of annexins on apoptotic cells is mediated by the annexin core domain. J Immunol, 2015-04-27;194(11):5233-42. 2015-04-27 [PMID: 25917090] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ANXA13
• Uniprot:
  • P27216()