Annexin A1 Antibody (OTI3A8) - Azide and BSA Free

Western Blot: Annexin A1 Antibody (OTI3A8) - Azide and BSA Free [NBP2-70174] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Annexin A1.

Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody (OTI3A8) - Azide and BSA Free [NBP2-70174] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Annexin A1.

Immunohistochemistry: Annexin A1 Antibody (OTI3A8) - Azide and BSA Free [NBP2-70174] - Staining of paraffin-embedded breast tissue using anti-Annexin A1 mouse monoclonal antibody.

Western Blot: Annexin A1 Antibody (OTI3A8) - Azide and BSA Free [NBP2-70174] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and ANXA1-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with

Immunohistochemistry: Annexin A1 Antibody (OTI3A8) - Azide and BSA Free [NBP2-70174] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Annexin A1 mouse monoclonal antibody.

Immunohistochemistry: Annexin A1 Antibody (OTI3A8) - Azide and BSA Free [NBP2-70174] - Staining of paraffin-embedded Kidney tissue using anti-Annexin A1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clone: OTI3A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Annexin A1 Antibody (OTI3A8) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse Annexin A1 Antibody (OTI3A8) - Azide and BSA Free (NBP2-02357) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ANXA1 (NP_000691) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:2000
• Theoretical MW: 38.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Annexin A1 Antibody (OTI3A8) - Azide and BSA Free

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I, also known as Annexin A1, belongs to the annexin family of calcium dependent phospholipid binding proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA1