Annexin A1 Antibody (OTI3A8)

Western Blot: Annexin A1 Antibody (OTI3A8) [NBP2-02357] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and ANXA1-Knockout hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-ANXA1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control (1:500).

Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody (OTI3A8) [NBP2-02357] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Annexin A1.

Immunohistochemistry-Paraffin: Annexin A1 Antibody (3A8) [NBP2-02357] - Staining of paraffin-embedded Kidney tissue using anti-Annexin A1 mouse monoclonal antibody.

Western Blot: Annexin A1 Antibody (3A8) [NBP2-02357] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Annexin A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Annexin A1.

Immunohistochemistry-Paraffin: Annexin A1 Antibody (3A8) [NBP2-02357] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Annexin A1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Annexin A1 Antibody (3A8) [NBP2-02357] - Staining of paraffin-embedded breast tissue using anti-Annexin A1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clone: OTI3A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.9 mg/ml

Annexin A1 Antibody (OTI3A8) Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse Annexin A1 Antibody (OTI3A8) (NBP2-02357) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ANXA1 (NP_000691) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Knockout Validated
  • Western Blot 1:2000
• Theoretical MW: 38.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Annexin A1 Antibody (OTI3A8)

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA1
• Entrez:
  • 301(Human)