Annexin A1 Antibody (CL0199) - Azide and BSA Free

Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.

Staining of A549 cells using the Anti-Annexin A1 monoclonal antibody) .

Analysis in human placenta and testis tissues using NBP3-43928 antibody. Corresponding Annexin A1 RNA-seq data are presented for the same tissues.

Staining of human fallopian tube shows strong positivity in glandular cells.

Staining of human lung shows strong nuclear positivity in a subset of pneumocytes and lymphoid cells.

Staining of human placenta shows strong immunoreactivity in the trophoblastic cells.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Annexin A1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0199
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Annexin A1 Antibody (CL0199) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Annexin A1 Antibody (CL0199) - Azide and BSA Free (NBP2-52875) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P04083, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANXA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Annexin A1 Antibody (CL0199) - Azide and BSA Free

• Annexin A1
• annexin I (lipocortin I)
• Annexin I
• Annexin-1
• ANX1
• ANXA1
• Calpactin II
• calpactin-2
• chromobindin-9
• Lipocortin I
• LPC1
• LPC1annexin-1
• p35
• Phospholipase A2 inhibitory protein

Background

Annexin I, also known as Annexin A1, belongs to the annexin family of calcium dependent phospholipid binding proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA1