ANKK1 Antibody

Images

 
Western Blot: ANKK1 Antibody [NBP1-28459] - Analysis of ANKK1 in Human Substantia Nigra lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ANKK1 Antibody [NBP1-28459] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ANKK1 Antibody Summary

Immunogen
Peptide with sequence C-SRQADPNLHEAEGKT corresponding to internal region according to NP_848605.1.
Epitope
C terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANKK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.3 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
Western blot: Approx. 85kDa band observed in Human Brain (Substantia nigra and Amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: Paraffin embedded Human Prostate
Publications
Read Publication using NBP1-28459.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ANKK1 Antibody

  • ankyrin repeat and kinase domain containing 1
  • ankyrin repeat and protein kinase domain-containing protein 1
  • EC 2.7.11
  • EC 2.7.11.1
  • PKK2
  • Protein kinase PKK2
  • SGK288
  • Sugen kinase 288
  • X-kinasesgK288

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and protein kinase superfamily involved in signal transduction pathways. This gene is closely linked to DRD2 gene (GeneID:1813) on chr 11, and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA, was originally associated with the DRD2 gene, however, later was determined to be located in exon 8 of ANKK1 gene (PMIDs: 18621654, 15146457), where it causes a nonconservative amino acid substitution. It is not clear if this gene plays any role in neuropsychiatric disorders previously associated with Taq1A RFLP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ANKK1 Antibody (NBP1-28459)(1)

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Bioinformatics

Gene Symbol ANKK1
Entrez
Uniprot