ANKK1 Antibody

Western Blot: ANKK1 Antibody [NBP1-28459] - Analysis of ANKK1 in Human Substantia Nigra lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ANKK1 Antibody [NBP1-28459] - (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ANKK1 Antibody Summary

• Description: Novus Biologicals Goat ANKK1 Antibody (NBP1-28459) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ANKK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SRQADPNLHEAEGKT corresponding to internal region according to NP_848605.1.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANKK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: Western blot: Approx. 85kDa band observed in Human Brain (Substantia nigra and Amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 35 kDa. This band was successfully blocked by incubation with the immunising peptide.
  IHC: Paraffin embedded Human Prostate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ANKK1 Antibody

• ankyrin repeat and kinase domain containing 1
• ankyrin repeat and protein kinase domain-containing protein 1
• EC 2.7.11
• EC 2.7.11.1
• PKK2
• Protein kinase PKK2
• SGK288
• Sugen kinase 288
• X-kinasesgK288

Background

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and protein kinase superfamily involved in signal transduction pathways. This gene is closely linked to DRD2 gene (GeneID:1813) on chr 11, and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA, was originally associated with the DRD2 gene, however, later was determined to be located in exon 8 of ANKK1 gene (PMIDs: 18621654, 15146457), where it causes a nonconservative amino acid substitution. It is not clear if this gene plays any role in neuropsychiatric disorders previously associated with Taq1A RFLP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ANKK1 Antibody (NBP1-28459)(1)

Publication using NBP1-28459

• Dick DM, Wang JC, Plunkett J et al. Family-based association analyses of alcohol dependence phenotypes across DRD2 and neighboring gene ANKK1. Alcohol Clin Exp Res 2007-10-01 [PMID: 17850642]

Bioinformatics

• Gene Symbol: ANKK1
• Entrez:
  • 255239(Human)
  • 93898(Mouse)
  • 306351(Rat)
• Uniprot:
  • NP_848605.1(Human)