Angiotensin I ELISA Kit (Colorimetric)

ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - Pooled human plasma was spiked with 500pg/ml of Angiotensin I, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was processed identically as a background control.

ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - A typical standard curve for Angiotensin I ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: Plasma,serum
• Standard Curve Range: 3.9-10,000 pg/ml
• Sensitivity: 4.3 pg/ml

Angiotensin I ELISA Kit (Colorimetric) Summary

• Specificity: Crossreactivity: Angiotensin I (100%), Angiotensin A (0.182%), Angiotensin II (0.083%), Angiotensin (1-12) (0.025%). Unaffected by Angiotensin (1-9), Angiotensin (1-7), Angiotensin III, Angiotensin IV, LVV-hemorphin 7, Bradykinin.
• Standard Curve Range: 3.9-10,000 pg/ml
• Sensitivity: 4.3 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 7.6% Medium Concentration %CV - 10.0% High Concentration %CV - 7.6%
• Intra-Assay: Low Concentration %CV - 10.0% Medium Concentration %CV - 7.3% High Concentration %CV - 8.3%
• Spike Recovery: Low Concentration %CV - 76.0%
  Medium - 90.0%
  High - 66.0%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AGT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Angiotensin I ELISA Kit (Colorimetric)

• alpha-1 antiproteinase, antitrypsin
• angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• angiotensinogen
• ANHU
• FLJ92595
• FLJ97926
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

The protein encoded by the Angiotensin I gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Angiotensin I ELISA Kit (NBP2-62134)(2)

Publications using NBP2-62134

• Zhang Y, Martin B, Spies MA et al. Renin and renin blockade have no role in complement activity Kidney international 2023-11-24 [PMID: 38008161] (ELISA)
• Firl DJ, Lassiter G, Hirose T et al. Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation Nature communications 2023-06-13 [PMID: 37311769] (ELISA)

FAQs for Angiotensin I ELISA Kit (NBP2-62134). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AGT