Angiotensin I ELISA Kit (Colorimetric)

ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - Pooled human plasma was spiked with 500pg/ml of Angiotensin I, serially diluted 1:2, and run in the assay. Unspiked pooled plasma was processed identically as a background control.

ELISA: Non-species specific Angiotensin I ELISA Kit (Colorimetric) [NBP2-62134] - A typical standard curve for Angiotensin I ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: Plasma,serum
• Standard Curve Range: 3.9-10,000 pg/ml
• Sensitivity: 4.3 pg/ml

Angiotensin I ELISA Kit (Colorimetric) Summary

• Specificity: Crossreactivity: Angiotensin I (100%), Angiotensin A (0.182%), Angiotensin II (0.083%), Angiotensin (1-12) (0.025%). Unaffected by Angiotensin (1-9), Angiotensin (1-7), Angiotensin III, Angiotensin IV, LVV-hemorphin 7, Bradykinin.
• Standard Curve Range: 3.9-10,000 pg/ml
• Sensitivity: 4.3 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Low Concentration %CV - 7.6% Medium Concentration %CV - 10.0% High Concentration %CV - 7.6%
• Intra-Assay: Low Concentration %CV - 10.0% Medium Concentration %CV - 7.3% High Concentration %CV - 8.3%
• Spike Recovery: Low Concentration %CV - 76.0%
  Medium - 90.0%
  High - 66.0%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AGT

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Angiotensin I ELISA Kit (Colorimetric)

• alpha-1 antiproteinase, antitrypsin
• angiotensin II
• angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• angiotensinogen
• ANHU
• FLJ92595
• FLJ97926
• pre-angiotensinogen
• serine (or cysteine) proteinase inhibitor
• Serpin A8
• SERPINA8angiotensin I

Background

The protein encoded by the Angiotensin I gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Angiotensin I ELISA Kit (NBP2-62134)(2)

Publications using NBP2-62134

• Zhang Y, Martin B, Spies MA et al. Renin and renin blockade have no role in complement activity Kidney international 2023-11-24 [PMID: 38008161] (ELISA)
• Firl DJ, Lassiter G, Hirose T et al. Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation Nature communications 2023-06-13 [PMID: 37311769] (ELISA)

FAQs for Angiotensin I ELISA Kit (NBP2-62134). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: AGT