Angiopoietin-like Protein 2/ANGPTL2 Antibody [Biotin]

ANGPTL2 expression is increased in stressed cardiomyocytes.(a–c) Representative western blot and quantitation of ANGPTL2 protein in hearts from WT mice 6 weeks after TAC or sham surgery (n=5 per group) (a) and 2 weeks after Angiotensin II (Ang II) or vehicle treatment (n=8 per group) (b). Analysis of ANGPTL2 and GFP protein from GFP+ cardiomyocytes and GFP− non-cardiomyocytes from Myh6-EGFP Tg mice (n=3 per group) (c). Hsc70 served as a loading control. Normalized values from controls (a,b) or GFP+ cardiomyocytes (c) were set to 1. (d,e) Quantitative RT-PCR analysis of Angptl2 expression in GFP+ and GFP– cells 6 weeks after TAC or sham surgery (d) and 2 weeks after Ang II or vehicle treatment (e) (n=3 per group). Values for respective controls were set to 1. (f,g) Relative ANGPTL2 transcript (f) and protein (g) levels in NRCMs assessed 6 or 12 h after Ang II (left in f,g) or Iso (right in f) treatment, with or without cyclosporine A (CsA) treatment. Values before treatment were set to 1. n=4–8 per group. (h) Relative ANGPTL2 transcript levels in NRCMs 8 h after Ang II treatment, with or without xestospongin C (Xes C) treatment. Values from control NRCMs were set to 1. n=8 per group. (i) Relative Angptl2 transcript levels in NRCMs overexpressing constitutively active NFAT (CA-NFAT). Values from control NRCMs were set to 1. n=3 per group. Data are means±s.e.m. Statistical significance was determined by Student's t test (a–e and i) or one-way ANOVA (f–h). *P<0.05, **P<0.01, †P<0.001 between groups. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27677409), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Angiopoietin-like Protein 2/ANGPTL2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Angiopoietin-like Protein 2/ANGPTL2
  Pro23-His493
  Accession # Q9R045
• Specificity: Detects mouse Angiopoietin-like Protein 2/ANGPTL2 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human ANGPTL2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) ANGPTL3 and rmAngiopoietin-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPTL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 2/ANGPTL2 Antibody [Biotin]

• angiopoietin-like 2
• Angiopoietin-like Protein 2
• angiopoietin-related protein 2
• ANGPTL2
• ANGRP2
• ARP2HARP
• MGC8889

Background

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-like Protein 2/ANGPTL2 Antibody (BAF1444)(9)

Publications using BAF1444

• H Horiguchi, T Kadomatsu, S Yumoto, T Masuda, K Miyata, S Yamamura, M Sato, J Morinaga, S Ohtsuki, H Baba, T Moroishi, Y Oike Tumor cell-derived ANGPTL2 promotes beta-catenin-driven intestinal tumorigenesis Oncogene, 2022-07-13;0(0):. 2022-07-13 [PMID: 35831580] (Western Blot, Mouse)
• H Osumi, H Horiguchi, T Kadomatsu, K Tashiro, J Morinaga, T Takahashi, K Ikeda, T Ito, M Suzuki, M Endo, Y Oike Tumor cell-derived angiopoietin-like protein 2 establishes a preference for glycolytic metabolism in lung cancer cells Cancer Sci., 2020-02-22;111(4):1241-1253. 2020-02-22 [PMID: 32012400] (Western Blot)
• Haruki Horiguchi, Tsuyoshi Kadomatsu, Ryoma Kurahashi, Chiaki Hara, Keishi Miyata, Masaya Baba et al. Dual functions of angiopoietin-like protein 2 signaling in tumor progression and anti-tumor immunity Genes & Development 12/1/2019 [PMID: 31727773]
• Haruki Horiguchi, Tsuyoshi Kadomatsu, Ryoma Kurahashi, Chiaki Hara, Keishi Miyata, Masaya Baba et al. Dual functions of angiopoietin-like protein 2 signaling in tumor progression and anti-tumor immunity Genes & Development 2019-12-01 [PMID: 31727773] (Western Blot, Mouse)
• J Zhao, Z Tian, T Kadomatsu, P Xie, K Miyata, T Sugizaki, M Endo, S Zhu, H Fan, H Horiguchi, J Morinaga, K Terada, T Yoshizawa, K Yamagata, Y Oike Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice J. Biol. Chem., 2017-11-30;0(0):. 2017-11-30 [PMID: 29191837] (Western Blot, Mouse)
• Z Tian, K Miyata, J Morinaga, H Horiguchi, T Kadomatsu, M Endo, J Zhao, S Zhu, T Sugizaki, M Sato, K Terada, T Okumura, T Murohara, Y Oike Circulating ANGPTL2 Levels Increase in Humans and Mice Exhibiting Cardiac Dysfunction Circ. J., 2017-09-08;0(0):. 2017-09-08 [PMID: 28890470] (Western Blot, Mouse)
• Zhe Tian, Keishi Miyata, Tsuyoshi Kadomatsu, Haruki Horiguchi, Hiroyuki Fukushima, Shugo Tohyama et al. ANGPTL2 activity in cardiac pathologies accelerates heart failure by perturbing cardiac function and energy metabolism Nature Communications 9/28/2016 [PMID: 27677409]
• Zhe Tian, Keishi Miyata, Tsuyoshi Kadomatsu, Haruki Horiguchi, Hiroyuki Fukushima, Shugo Tohyama et al. ANGPTL2 activity in cardiac pathologies accelerates heart failure by perturbing cardiac function and energy metabolism Nature Communications 2016-09-28 [PMID: 27677409] (Western Blot, Mouse, Transgenic Mouse)
• Nakamura T, Okada T, Endo M, Kadomatsu T, Taniwaki T, Sei A, Odagiri H, Masuda T, Fujimoto T, Nakamura T, Oike Y, Mizuta H Angiopoietin-like protein 2 induced by mechanical stress accelerates degeneration and hypertrophy of the ligamentum flavum in lumbar spinal canal stenosis. PLoS ONE, 2014-01-17;9(1):e85542. 2014-01-17 [PMID: 24465594] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ANGPTL2
• Uniprot:
  • Q9R045()