ANAPC13 Antibody - BSA Free

Western Blot: ANAPC13 Antibody - BSA Free [NBP1-76261] - Western blot analysis of ANAPC13 in Jurkat cell tissue lysate with ANAPC13 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Western Blot: ANAPC13 Antibody [NBP1-76261] - Western blot analysis with 25ng of recombinant ANAPPC13 protein with ANAPC13 antibody at 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: ANAPC13 Antibody - BSA Free [NBP1-76261] - Immunofluorescence of ANAPC13 in Jurkat cells with ANAPC13 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ANAPC13 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ANAPC13 Antibody - BSA Free (NBP1-76261) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13. The immunogen is located within the last 50 amino acids of APC13. Amino Acid Squence: PLNELPEPEQDNGGTTE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC13
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ANAPC13 Peptide (NBP1-76261PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ANAPC13 Antibody - BSA Free

• anaphase promoting complex subunit 13
• anaphase-promoting complex subunit 13
• APC13SWM1
• cyclosome subunit 13
• Cylosome subunit 13
• DKFZP566D193
• homolog of yeast Swm1

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANAPC13
• Uniprot:
  • NP_056206(Human)