Rat Amylin ELISA Kit (Colorimetric)

ELISA: Rat Amylin ELISA Kit (Colorimetric) [NBP2-76735] - Samples were spiked with high concentrations of Rat Amylin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Amylin ELISA Kit (Colorimetric) [NBP2-76735] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.5 - 4000pg/mL
• Sensitivity: 37.5pg/mL

Rat Amylin ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 62.5 - 4000pg/mL
• Sensitivity: 37.5pg/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: CV% < 4.73
• Intra-Assay: CV% < 5.83
• Spike Recovery: 85-109%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IAPP

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Amylin ELISA Kit (Colorimetric)

• AMYLIN
• DAPamylin
• Diabetes-associated peptide
• IAP
• Insulinoma amyloid peptide
• Islet amyloid polypeptide (diabetes-associated peptide; amylin)
• islet amyloid polypeptide

Background

Amylin is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell death in particular cultured cells, an effect that may be relevant to the development of type II diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Amylin ELISA Kit (NBP2-76735)(3)

Publications using NBP2-76735

• Aranäs C, Edvardsson CE, Zentveld L et Al. The combination of a glucagon-like peptide-1 and amylin receptor agonists reduces alcohol consumption in both male and female rats Acta Neuropsychiatr 2024-12-06 [PMID: 39639536] (ELISA)
• Tang S, Fang C, Liu Y et al. Anti-obesity and Anti-diabetic Effect of Ursolic Acid against Streptozotocin/High Fat Induced Obese in Diabetic Rats Journal of oleo science 2022-01-14 [PMID: 35034940]
• Asra Iftikhar, Bilal Aslam, Maryam Iftikhar, Wafa Majeed, Mehwish Batool, Bushra Zahoor, Naseem Amna, Hareem Gohar, Iqra Latif Effect of Caesalpinia bonduc Polyphenol Extract on Alloxan-Induced Diabetic Rats in Attenuating Hyperglycemia by Upregulating Insulin Secretion and Inhibiting JNK Signaling Pathway Oxidative Medicine and Cellular Longevity 2020-03-17 [PMID: 32256963]

FAQs for Amylin ELISA Kit (NBP2-76735). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IAPP