AMPK gamma 1 Antibody - BSA Free

Western Blot: AMPK gamma 1 Antibody [NBP1-50668] - Mouse heart tissue lysate.

Immunohistochemistry-Paraffin: AMPK gamma 1 Antibody [NBP1-50668] - Human breast cancer tissue (left) and human testis tissue (right) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.25 mg/ml

AMPK gamma 1 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide selected from the center of human PRKAG1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAG1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.25 ug/mL
  • Immunohistochemistry 2.5 - 5 ug/mL
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 0.5 - 2.5 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Affinity purified

Alternate Names for AMPK gamma 1 Antibody - BSA Free

• 5'-AMP-activated protein kinase subunit gamma-1
• 5'-AMP-activated protein kinase, gamma-1 subunit
• AMPK gamma 1
• AMPK gamma-1 chain
• AMPK gamma1
• AMPK subunit gamma-1
• AMPKG
• AMPKG1
• MGC8666
• PRKAG1
• protein kinase, AMP-activated, gamma 1 non-catalytic subunit

Background

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for AMPK gamma 1 Antibody (NBP1-50668) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Angel Lin WB Mouse 04/01/2013

Summary

• Application: Western Blot
• Sample Tested: Brain, germ cells, Repro proteins
• Species: Mouse

Bioinformatics

• Gene Symbol: PRKAG1
• Entrez:
  • 5571(Human)
• Uniprot:
  • P54619(Human)