AMPK gamma 1 Antibody

Images

 
Western Blot: AMPK gamma 1 Antibody [NBP1-50668] - Mouse heart tissue lysate.
Immunohistochemistry-Paraffin: AMPK gamma 1 Antibody [NBP1-50668] - Human breast cancer tissue (left) and human testis tissue (right) reacted with the primary antibody, which was peroxidase-conjugated to the secondary ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.25 mg/ml

Order Details

AMPK gamma 1 Antibody Summary

Immunogen
Synthetic peptide selected from the center of human PRKAG1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKAG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
The antibody can be used for ELISA (0.25 ug/ml), Western blotting (0.5 - 2.5 ug/ml) and Immunohistochemistry (2.5-5.0 ug/ml).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-50668 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMPK gamma 1 Antibody

  • 5'-AMP-activated protein kinase subunit gamma-1
  • 5'-AMP-activated protein kinase, gamma-1 subunit
  • AMPK gamma-1 chain
  • AMPK gamma1
  • AMPK subunit gamma-1
  • AMPKG
  • MGC8666
  • protein kinase, AMP-activated, gamma 1 non-catalytic subunit

Background

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AMPK gamma 1 Antibody (NBP1-50668) (0)

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Review for AMPK gamma 1 Antibody (NBP1-50668) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-50668:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot AMPK gamma 1 NBP1-50668
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reviewed by:
Angel Lin
WB Mouse 04/01/2013
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Summary

ApplicationWestern Blot
Sample TestedBrain, germ cells, Repro proteins
SpeciesMouse
CommentsAntibody worked well. Very simple process

Blocking

Blocking Details5% Non fat milk in TBST at RT for 1 hr

Primary Anitbody

Dilution Ratio1:500 in 5% non fat milk in TBST, 4C O/N

Secondary Antibody

Secondary Descriptionanti-rabbit HRP
Secondary Concentration1:5000

Details

Detection NotesECL prime, 10 min exposure

Comments

CommentsAntibody worked well. Very simple process

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for AMPK gamma 1 Antibody (NBP1-50668) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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1
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Angel Lin
04/01/2013
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PRKAG1
Entrez
Uniprot