AMPK gamma 1 Antibody - Azide and BSA Free

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P] - Analysis of PRKAG1 expression in A-431.

Immunocytochemistry/Immunofluorescence: AMPK gamma 1 Antibody [H00005571-D01P] - Analysis of antibody to PRKAG1 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: AMPK gamma 1 Antibody [H00005571-D01P] - Analysis of PRKAG1 expression in transfected 293T cell line by PRKAG1 polyclonal antibody.Lane 1: PRKAG1 transfected lysate(28.30 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AMPK gamma 1 Antibody - Azide and BSA Free Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PRKAG1 (NP_997626.1, 1 a.a. - 247 a.a.) full-length human protein. MLTITDFINILHRYYKSALVQIYELEEHKIETWREVYLQDSFKPLVCISPNASLFDAVSSLIRNKIHRLPVIDPESGNTLYILTHKRILKFLKLFITEFPKPEFMSKSLEELQIGTYANIAMVRTTTPVYVALGIFVQHRVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLDVSVTKALQHRSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVDENDVVKGIVSLSDILQALVLTGGEKKP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAG1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: It has been used for WB and Functional.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AMPK gamma 1 Antibody - Azide and BSA Free

• 5'-AMP-activated protein kinase subunit gamma-1
• 5'-AMP-activated protein kinase, gamma-1 subunit
• AMPK gamma 1
• AMPK gamma-1 chain
• AMPK gamma1
• AMPK subunit gamma-1
• AMPKG
• AMPKG1
• MGC8666
• PRKAG1
• protein kinase, AMP-activated, gamma 1 non-catalytic subunit

Background

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1), is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpah subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAG1