AMPK beta 2/PRKAB2 Antibody (2G9)

Western Blot: AMPK beta 2/PRKAB2 Antibody (2G9) [H00005565-M01] - PRKAB2 monoclonal antibody (M01), clone 2G9 Analysis of PRKAB2 expression in Hela.

Sandwich ELISA: AMPK beta 2/PRKAB2 Antibody (2G9) [H00005565-M01] - Detection limit for recombinant GST tagged PRKAB2 is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, S-ELISA
• Clone: 2G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

AMPK beta 2/PRKAB2 Antibody (2G9) Summary

• Immunogen: PRKAB2 (AAH53610, 1 a.a. ~ 272 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI
• Specificity: PRKAB2 - protein kinase, AMP-activated, beta 2 non-catalytic subunit
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKAB2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AMPK beta 2/PRKAB2 Antibody (2G9)

• 5'-AMP-activated protein kinase, beta-2 subunit
• AMPK beta 2
• AMPK beta 2,5'-AMP-activated protein kinase subunit beta-2
• AMPK beta-2 chain
• AMPK subunit beta-2
• MGC61468
• PRKAB2
• protein kinase, AMP-activated, beta 2 non-catalytic subunit

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAB2
• Entrez:
  • 5565(Human)
• OMIM:
  • 602741(Human)
• Uniprot:
  • AAH53610(Human)