AMPK alpha 1 [p Thr172] Antibody

Product Discontinued

AMPK alpha 1 [p Thr172] Antibody Summary

• Immunogen: A synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha 1 protein from human origin. It is identical among human, mouse, rat, chicken, bovine, dog, and insect species.
• Modification: p Thr172
• Localization: Cytoplasmic
• Specificity: This antibody recognizes AMPK alpha with a phosphorylated site of Thr 172. It does not cross-react with non-phosphospecific peptide.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.01-0.1 ug/ml
  • Immunoprecipitation 2-5 ug/ml
  • Western Blot 0.1-1 ug/ml
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile TBS (pH 7.2) with antibody stabilizer
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMPK alpha 1 [p Thr172] Antibody

• AMP -activate kinase alpha 1 subunit
• AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
• AMPK alpha 1
• AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
• AMPK subunit alpha-1
• AMPK
• AMPK1
• AMPKa1
• EC 2.7.11
• EC 2.7.11.1
• MGC33776
• MGC57364
• PRKAA1
• protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPK alpha 1 Antibody (NBP1-74502)(4)

Publications using NBP1-74502

• Harun-Or-Rashid M, Pappenhagen N, Zubricky R et al. MCT2 overexpression rescues metabolic vulnerability and protects retinal ganglion cells in two models of glaucoma Neurobiol. Dis. 2020-05-15 [PMID: 32422282] (WB, Mouse)
• Allen SA, Datta S, Sandoval J et al. Cetylpyridinium chloride is a potent AMP-activated kinase (AMPK) inducer and has therapeutic potential in cancer Mitochondrion 2019-10-22 [PMID: 31654752] (Mouse)
• Harun-Or-Rashid M, Inman DM. Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma. J Neuroinflammation. 2018-11-13 [PMID: 30424795] (WB, Mouse)
• Pesi R, Petrotto E, Colombaioni L et al. Cytosolic 5'-Nucleotidase II Silencing in a Human Lung Carcinoma Cell Line Opposes Cancer Phenotype with a Concomitant Increase in p53 Phosphorylation Int J Mol Sci 2018-07-20 [PMID: 30037008] (WB, Human)

FAQs for AMPK alpha 1 Antibody (NBP1-74502). (Showing 1 - 1 of 1 FAQs).

1. I have questions about your product, NBP1-74502 (AMPK alpha 1 p Thr172 antibody). I’d like to detect p-AMPK alpha 1 (Thr172) by Western blotting. I can see an image of Western blotting in your product sheet, wherein, the sample is from HEK293. I’d like to know how much protein is loaded in this case? Please tell me the blocking condition and what solution is used to dilute the antibody?
   • For the western of product NBP1-74502, we loaded 30ug of lysate in the gel. We blocked the blot with 5% non-fat dry milk and 1% BSA in TBST (0.1% Tween) for 1 hour at room temperature. The primary antibody was diluted in the blocking buffer.

Bioinformatics

• Gene Symbol: PRKAA1
• Entrez:
  • 5562(Human)
• Uniprot:
  • Q13131()