AMPD2 Antibody (2F5)

Western Blot: AMPD2 Antibody (2F5) [H00000271-M01A] - Metformin regulates AMPD2 activity in human hepatocytes.Over-expression of AMPD2 down-regulates the activation of AMPK. Transduction of HepG2 cells with lentiviral particles codifying for the isoform 2 of AMPD2 results in significantly higher levels of AMPD2 protein expression as well as AMPD activity. This is paralleled with reduced levels Thr172 pAMPK expression as well as of their target genes ECH1 and Ser79 pACC. *p<0.05. Image collected and cropped by CiteAb from the following publication (www.dx.plos.org/10.1371/journal.pone.0048801) licensed under a CC-BY license.

Western Blot: AMPD2 Antibody (2F5) [H00000271-M01A] - AMPD2 monoclonal antibody (M01A), clone 2F5. Analysis of AMPD2 expression in PC-12.

Western Blot: AMPD2 Antibody (2F5) [H00000271-M01A] - AMPD2 monoclonal antibody (M01A), clone 2F5 Analysis of AMPD2 expression in Hela S3 NE.

Western Blot: AMPD2 Antibody (2F5) [H00000271-M01A] - AMPD2 monoclonal antibody (M01A), clone 2F5. Analysis of AMPD2 expression in Raw 264.7.

Western Blot: AMPD2 Antibody (2F5) [H00000271-M01A] - AMPD2 monoclonal antibody (M01A), clone 2F5. Analysis of AMPD2 expression in NIH/3T3.

Western Blot: AMPD2 Antibody (2F5) [H00000271-M01A] - Analysis of AMPD2 expression in transfected 293T cell line by AMPD2 monoclonal antibody (M01A), clone 2F5.Lane 1: AMPD2 transfected lysate(92.1 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

AMPD2 Antibody (2F5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AMPD2 (NP_631895, 86 a.a. ~ 185 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ISQDVKLEPDILLRAKQDFLKTDSDSDLQLYKEQGEGQGDRSLRERDVLEREFQRVTISGEEKCGVPFTDLLDAAKSVVRALFIREKYMALSLQSFCPTT
• Specificity: AMPD2 - adenosine monophosphate deaminase 2 (isoform L) (2F5)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AMPD2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reactivity Notes

Squirrel reactivity reported in scientific literature (PMID: 25856396). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AMPD2 Antibody (2F5)

• adenosine monophosphate deaminase 2 (isoform L)
• adenosine monophosphate deaminase 2
• AMP deaminase 2
• AMP deaminase isoform L
• AMPD
• EC 3.5.4.6

Background

Adenosine monophosphate deaminase-2 (EC 3.5.4.6) catalyzes the deamination of AMP to IMP and plays an important role inthe purine nucleotide cycle.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPD2 Antibody (H00000271-M01A)(2)

Publications using H00000271-M01A

• Lanaspa MA, Epperson LE, Li N et al. Opposing Activity Changes in AMP Deaminase and AMP-Activated Protein Kinase in the Hibernating Ground Squirrel PLoS ONE 2015-04-10 [PMID: 25856396] (WB, Squirrel)
• Lanaspa MA, Cicerchi C, Garcia G et al. Counteracting Roles of AMP Deaminase and AMP Kinase in the Development of Fatty Liver PLoS One 2012-01-01 [PMID: 23152807] (WB, Rat)

Bioinformatics

• Gene Symbol: AMPD2
• Entrez:
  • 271(Human)
• Uniprot:
  • NP_631895(Human)