Aminopeptidase A/ENPEP Antibody (6C3) - BSA Free

Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Detection of Aminopeptidase A/ENPEP in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse CD11b (Catalog # FAB1124P) and Rat Anti-Mouse Aminopeptidase A/ENPEP Monoclonal Antibody (Catalog # NBP1-43281, top panel) or isotype control antibody (Catalog # MAB006, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).

Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Staining of BALB/c bone marrow cells with 0.5 ug of Rat IgG2a K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse BP-1 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Cells in the lymphocyte gate were used

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: 6C3
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Aminopeptidase A/ENPEP Antibody (6C3) - BSA Free Summary

• Description: Novus Biologicals Rat Aminopeptidase A/ENPEP Antibody (6C3) - BSA Free (NBP1-43281) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was Aminopeptidase A/APA.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ENPEP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Aminopeptidase A/ENPEP Antibody (6C3) - BSA Free

• Aminopeptidase A
• APA
• AP-A
• CD249 antigen
• CD249
• Differentiation antigen gp160
• EAP
• EC 3.4.11
• EC 3.4.11.7
• ENPEP
• glutamyl aminopeptidase (aminopeptidase A)
• glutamyl aminopeptidase
• gp160

Background

The 6C3 monoclonal antibody reacts with mouse Ly-51 and 6C3 antigen. LY-51 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of LY-51 expression on pre-B cells can be upregulated in the presence of IL-7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENPEP
• Uniprot:
  • Q07075(Human)