![Western Blot: AMIGO Antibody [NBP2-81780] - Analysis of AMIGO in HeLa cell lysate with AMIGO antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/AMIGO-Antibody-Western-Blot-NBP2-81780-img0001.jpg "Western Blot: AMIGO Antibody [NBP2-81780] - Analysis of AMIGO in HeLa cell lysate with AMIGO antibody at (A) 1 and (B) 2 ug/ml.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit AMIGO Antibody - BSA Free (NBP2-81780) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | AMIGO antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AMIGO. The immunogen is located within the last 50 amino acids of AMIGO. Amino Acid Squence: AGPGQGQNGKLKPGNTLPV |
| Specificity | AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AMIGO1 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry AMIGO3 Antibody (371301) [Unconjugated]](https://images.novusbio.com/images2/AMIGO3_MAB2375_Immunohistochemistry_6971.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for AMIGO Antibody (NBP2-81780)Find related products by research area.
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| Gene Symbol | AMIGO1 |
![Immunocytochemistry/ Immunofluorescence: AMIGO Antibody - BSA Free [NBP2-81780] - Immunofluorescence of AMIGO in human brain tissue with AMIGO antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81780_rabbit-polyclonal-amigo-antibody-2711202419293882.jpg "Immunocytochemistry/ Immunofluorescence: AMIGO Antibody - BSA Free [NBP2-81780] - Immunofluorescence of AMIGO in human brain tissue with AMIGO antibody at 20 ug/mL.")
![Immunohistochemistry: AMIGO Antibody - BSA Free [NBP2-81780] - Immunohistochemistry of AMIGO in human brain tissue with AMIGO antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81780_rabbit-polyclonal-amigo-antibody-2711202419183812.jpg "Immunohistochemistry: AMIGO Antibody - BSA Free [NBP2-81780] - Immunohistochemistry of AMIGO in human brain tissue with AMIGO antibody at 5 ug/mL.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")