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AMIGO2 Antibody (290728)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
290728
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

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MAB2374-SP
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AMIGO2 Antibody (290728) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse AMIGO2
Met39-Ala391
Accession # Q80ZD9
Specificity
Detects mouse AMIGO2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human (rh) AMIGO2 and no cross-reactivity with rhAMIGO1 or recombinant mouse AMIGO3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
AMIGO2
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 3
using
MAB2374 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMIGO2 Antibody (290728)

  • adhesion molecule with Ig-like domain 2
  • ALI1amphoterin induced gene 2
  • alivin 1
  • Alivin-1
  • AMIGO2
  • AMIGO-2
  • DEGAamphoterin-induced protein 2
  • differentially expressed in gastric adenocarcinoma
  • Differentially expressed in gastric adenocarcinomas
  • transmembrane protein AMIGO2

Background

AMIGO2 is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (LRRs) and one Ig domain in the extracellular region. AMIGO2 is expressed primarily in cerebellum, retina, liver, and lung. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) AMIGO-1 Antibody. (D) Composite.
Immunocytochemistry/Immunofluorescence: AMIGO Antibody (S86-36) [NBP2-22413] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36 (NBP2-22413). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody (NBP2-22413) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) AMIGO-1 Antibody (D) Composite.
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Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Rabbit blood vessel. Image from verified customer review.
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AMIGO2 Antibody
(290728)
MAB2374
AMIGO2 Antibody (290728)
Species: Hu, Mu
Applications: WB

Publications for AMIGO2 Antibody (MAB2374) (0)

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Reviews for AMIGO2 Antibody (MAB2374) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using MAB2374:
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(1)
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Images Ratings Applications Species Date Details
  1
reviewed by:
Verified Customer
 IHC-P Human 07/20/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman colon cancer tissue
SpeciesHuman
LotVLF015051

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
Immunocytochemistry AMIGO2 MAB2374
Enlarge 		5
reviewed by:
Verified Customer
 ICC Mouse 10/05/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedprimary astrocytes
SpeciesMouse
LotVLF015051

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Immunohistochemistry-Paraffin AMIGO2 MAB2374
Enlarge
 Verified Customer
07/20/2022
Application: IHC-P
Species: Human
read this review
Immunocytochemistry AMIGO2 MAB2374
Enlarge
 Verified Customer
10/05/2017
Application: ICC
Species: Mouse
read this review

Bioinformatics

Gene Symbol AMIGO2
Uniprot