AMIGO3 Antibody (371301) [Unconjugated]

AMIGO3 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 25 µg/mL Rat Anti-Mouse AMIGO3 Monoclonal Antibody (Catalog # MAB2375) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 371301
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

AMIGO3 Antibody (371301) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse AMIGO3
  Thr20-Pro378
  Accession # Q8C2S7
• Specificity: Detects mouse AMIGO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse AMIGO2, recombinant human (rh) AMIGO1, or rhAMIGO2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: AMIGO3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMIGO3 Antibody (371301) [Unconjugated]

• adhesion molecule with Ig-like domain 3
• ALI3
• Alivin-3
• AMIGO3
• AMIGO-3
• amphoterin-induced gene and ORF 3
• amphoterin-induced protein 3
• KIAA1851
• MGC120552

Background

AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AMIGO3
• Uniprot:
  • Q8C2S7()