ALS2CR2 Antibody

Images

 
Western Blot: ALS2CR2 Antibody [NB100-1181] - HEK293 overexpressing ILPIP and probed with (mock transfection in first lane).
Immunohistochemistry: ALS2CR2 Antibody [NB100-1181] - (3.8ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: ALS2CR2 Antibody [NB100-1181] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ALS2CR2 Antibody Summary

Immunogen
Peptide with sequence CDFPDEKDSYWEF corresponding to C-Terminus according to NP_061041.2, AAF71042.1.
Specificity
This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STRADB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 35 kDa band observed in human heart, liver and muscle lysates (calculated MW of 47.0 kDa band according to NP_061041.2 (alpha isoform) and 31.2 kDa band according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human heart shows staining of myocardial fibres in transverse section.
Positive Control
Liver Lysate (NB820-59232)
Heart Lysate (NB820-59217)
ALS2CR2 Lysate (NBL1-07490)
Publications
Read Publication using NB100-1181.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ALS2CR2 Antibody

  • ALS2CR2candidate 2
  • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
  • CALS-21MGC102916
  • ILP-interacting protein
  • ILPIPSTRAD beta
  • Pseudokinase ALS2CR2
  • STE20-related kinase adaptor beta

Background

Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, is connected to transferase/kinase activity and ATP binding, it has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family. Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1beta converting enzyme of Fas-induced apotosis. This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival, suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALS2CR2 Antibody (NB100-1181)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STRADB
Entrez
Uniprot