ALS2CR2 Antibody

Western Blot: ALS2CR2 Antibody [NB100-1181] - HEK293 overexpressing ILPIP and probed with (mock transfection in first lane).

Immunocytochemistry/Immunofluorescence: ALS2CR2 Antibody [NB100-1181] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunohistochemistry: ALS2CR2 Antibody [NB100-1181] - Staining of paraffin embedded Human Heart. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: ALS2CR2 Antibody [NB100-1181] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ALS2CR2 Antibody Summary

• Description: Novus Biologicals Goat ALS2CR2 Antibody (NB100-1181) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-ALS2CR2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CDFPDEKDSYWEF corresponding to C-Terminus according to NP_061041.2, AAF71042.1.
• Specificity: This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STRADB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 3 - 5 ug/mL
  • Immunohistochemistry-Paraffin 3 - 5 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 35 kDa band observed in human heart, liver and muscle lysates (calculated MW of 47.0 kDa band according to NP_061041.2 (alpha isoform) and 31.2 kDa band according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human heart shows staining of myocardial fibres in transverse section.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  Human Heart Whole Tissue Lysate (Adult Whole Normal)
  ALS2CR2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALS2CR2 Antibody

• ALS2CR2candidate 2
• Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein
• CALS-21MGC102916
• ILP-interacting protein
• ILPIPSTRAD beta
• Pseudokinase ALS2CR2
• STE20-related kinase adaptor beta

Background

Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, is connected to transferase/kinase activity and ATP binding, it has recently been shown to interact with XIAP, a member of the IAP (Inhibitor of Apoptosis) protein family. Together, these two proteins synergistically activate the TAK1/JNK1 signal transduction pathway, which acts to protect against the interleukin-1beta converting enzyme of Fas-induced apotosis. This protein was independently isolated and characterized as polyploidy-associated protein kinase (PAPK), a member of the Ste20/germinal center kinase family that modulates cytoskeletal organization and cell survival, suggesting that its protective role may be a function of its kinase activity. Although two mRNA sequences have been isolated, only the shorter isoform has been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALS2CR2 Antibody (NB100-1181)(1)

Publication using NB100-1181

• Sanna MG, da Silva Correia J, Luo Y et al. ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis. J Biol Chem 2002-08-23 [PMID: 12048196]

Bioinformatics

• Gene Symbol: STRADB
• Entrez:
  • 55437(Human)
• Uniprot:
  • AAF71042(Human)