NIF3L1 Antibody

Images

 
Western Blot: NIF3L1 Antibody [NB100-1185] - Analysis of NIF3L1 in Human Heart lysate using this antibody at 1 ug/ ml.
Immunohistochemistry: NIF3L1 Antibody [NB100-1185] - IHC staining of NIF3L1 in paraffin embedded Human Breast using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NIF3L1 Antibody Summary

Immunogen
Peptide with sequence C-ILSETDRDPLQVV corresponding to C-Terminus according to NP_001129511.1, NP_068596.2.
Specificity
This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1; NP_068596.2) encode the same isoform.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NIF3L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 1 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 40 kDa band observed in human heart and human testis extracts (predicted MW of approx. 39 kDa band according to NP_068596). IHC-P: Human breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells.
Positive Control
NIF3L1 Lysate (NBL1-13642)
Heart Lysate (NB820-59217)
Testis Lysate (NB820-59266)
Publications
Read Publication using NB100-1185.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NIF3L1 Antibody

  • ALS2CR1
  • candidate 1
  • MDS015
  • NIF3 NGG1 interacting factor 3-like 1 (S. pombe)
  • NIF3-like protein 1
  • S.pombe homolog)-like 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NIF3L1 Antibody (NB100-1185)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NIF3L1
Entrez
Uniprot