alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

Western Blot: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal diluted at 1:200. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (NBP2-43559) diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Mouse brain. NAGLU antibody [N3C1], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.

Western Blot: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000

Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP2-19465] - Human lung adenocarcinoma, using NAGLU antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha-N-acetylglucosaminidase/NAGLU. The exact sequence is proprietary.
• Predicted Species: Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAGLU
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for alpha-N-acetylglucosaminidase/NAGLU Antibody - BSA Free

• alphaNacetylglucosaminidase
• alpha-N-acetylglucosaminidase
• EC 3.2.1.50
• MPS3B
• MPS-IIIB
• N-acetyl-alpha-glucosaminidase
• N-acetylglucosaminidase, alpha
• NAG
• NAGalpha-N-acetylglucosaminidase
• NAGLU
• UFHSD
• UFHSD1

Background

This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: NAGLU
• Uniprot:
  • P54802()