alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3)

Images

 
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis using AMACR mouse mAb against Jurkat (1) and LNCaP (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Confocal immunofluorescence analysis of LNCaP cells using AMACR mouse mAb (green). Red: Actin filaments have ...read more
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis of human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic ...read more
Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) [NBP1-28884] - Analysis of human brain cerebellum using AMACR mouse mAb.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2A10F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3) Summary

Immunogen
Purified recombinant fragment of human AMACR expressed in E. Coli.
Specificity
Specific for AMACR.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AMACR
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
alpha-Methylacyl-CoA Racemase/AMACR Knockout HeLa Cell Lysate

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody (2A10F3)

  • 2-methylacyl-CoA racemase
  • alpha-Methylacyl-CoA Racemase
  • alphaMethylacylCoARacemase
  • AMACR
  • AMACRD
  • CBAS4
  • EC 5.1.99.4
  • RACE
  • RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (NBP1-28884) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol AMACR