alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4)

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4) [NBP2-34471] - Western Blot Analysis of Human Kidney tissue lysate using alpha-Methylacyl-CoA Racemase/AMACR Rabbit Monoclonal Antibody (13H4).

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4) [NBP2-34471] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with alpha-Methylacyl-CoA Racemase/AMACR Rabbit Monoclonal Antibody (13H4)

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4) [NBP2-34471] - Formalin-fixed, paraffin-embedded human prostate carcinoma (20X) stained with AMACR / p504S Rabbit MAb (13H4).

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4) [NBP2-34471] - Formalin-fixed, paraffin-embedded human prostate carcinoma (10X) stained with AMACR / p504S Rabbit MAb (13H4).

Immunohistochemistry-Paraffin: alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4) [NBP2-34471] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with alpha-Methylacyl-CoA Racemase/AMACR Rabbit Monoclonal Antibody (13H4)

Product Discontinued

alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Full length human recombinant alpha-Methylacyl-CoA Racemase/AMACR protein
• Localization: Cytoplasmic
• Marker: Prostate Cancer Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AMACR
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:25-1:50
  • Western Blot
• Application Notes: Immunohistology (Formalin-fixed) (1:50-1:100 for 30-60 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Culture medium
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for alpha-Methylacyl-CoA Racemase/AMACR Antibody (13H4)

• 2-methylacyl-CoA racemase
• alpha-Methylacyl-CoA Racemase
• alphaMethylacylCoARacemase
• AMACR
• AMACRD
• CBAS4
• EC 5.1.99.4
• RACE
• RM

Background

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-Methylacyl-CoA Racemase/AMACR Antibody (NBP2-34471)(1)

Publication using NBP2-34471

• Shapovalova M, Davydova J, Henzler C et al. Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget 2018-11-30 [PMID: 30613352] (IHC-P, Human)

Bioinformatics

• Gene Symbol: AMACR
• Uniprot:
  • Q9UHK6(Human)