Alpha-mannosidase II Antibody

Images

 
Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).
Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - Analysis of alpha-mannosidase II antibody [NB100-93414] (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Alpha-mannosidase II Antibody Summary

Immunogen
Peptide with sequence C-KISSDIKSQNR corresponding to internal region according to NP_002363.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MAN2A1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 150 kDa band observed in human placenta lysates (calculated MW of 131 kDa band according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293. An anonymous customer has reported positive results on human dermal fibroblasts.
Control
Alpha-mannosidase II Overexpression Lysate
Publications
Read Publication using NB100-93414.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Alpha-mannosidase II Antibody

  • alpha-mannosidase 2
  • AMan II
  • EC 3.2.1
  • EC 3.2.1.114
  • Golgi alpha-mannosidase II
  • golgi integral membrane protein 7
  • GOLIM7
  • Man II
  • MANA2MANII
  • Mannosidase alpha class 2A member 1
  • mannosidase, alpha type II
  • mannosidase, alpha, class 2A, member 1
  • Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Background

This gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alpha-mannosidase II Antibody (NB100-93414)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MAN2A1
Entrez
Uniprot