Alpha-mannosidase II Antibody

Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - HEK293 overexpressing Man2A1 and probed with this antibody (mock transfection in first lane).

Western Blot: Alpha-mannosidase II Antibody [NB100-93414] - Analysis of alpha-mannosidase II antibody [NB100-93414] (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Alpha-mannosidase II Antibody Summary

• Immunogen: Peptide with sequence C-KISSDIKSQNR corresponding to internal region according to NP_002363.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAN2A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in human placenta lysates (calculated MW of 131 kDa band according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98 kDa is observed. This band is not observed in the non-transfected HEK293. An anonymous customer has reported positive results on human dermal fibroblasts.
• Control:

  Alpha-mannosidase II Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Alpha-mannosidase II Antibody

• alpha-mannosidase 2
• AMan II
• EC 3.2.1
• EC 3.2.1.114
• Golgi alpha-mannosidase II
• golgi integral membrane protein 7
• GOLIM7
• Man II
• MANA2MANII
• Mannosidase alpha class 2A member 1
• mannosidase, alpha type II
• mannosidase, alpha, class 2A, member 1
• Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Background

This gene encodes a protein which is a member of family 38 of the glycosyl hydrolases. The protein is located in the Golgi and catalyzes the final hydrolytic step in the asparagine-linked oligosaccharide (N-glycan) maturation pathway. Mutations in the mouse homolog of this gene have been shown to cause a systemic autoimmune disease similar to human systemic lupus erythematosus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alpha-mannosidase II Antibody (NB100-93414)(1)

Publication using NB100-93414

• Green RS, Stone EL, Tenno M et al. Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis. Immunity 2007-08-01 [PMID: 17681821]

Bioinformatics

• Gene Symbol: MAN2A1
• Entrez:
  • 4124(Human)
• Uniprot:
  • Q16706(Human)