Alpha-mannosidase II Overexpression Lysate

Western Blot: Alpha-mannosidase II Overexpression Lysate (Adult Normal) [NBL1-12834] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alpha-mannosidase II.

• Reactivity: Hu
• Applications: WB

Alpha-mannosidase II Overexpression Lysate Summary

• Description: Transient overexpression lysate of mannosidase, alpha, class 2A, member 1 (MAN2A1) The lysate was created in HEK293T cells, using Plasmid ID RC220186 and based on accession number NM_002372. The protein contains a C-MYC/DDK Tag.
• Gene: MAN2A1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Alpha-mannosidase II Overexpression Lysate

• alpha-mannosidase 2
• AMan II
• EC 3.2.1
• EC 3.2.1.114
• Golgi alpha-mannosidase II
• golgi integral membrane protein 7
• GOLIM7
• Man II
• MANA2MANII
• Mannosidase alpha class 2A member 1
• mannosidase, alpha type II
• mannosidase, alpha, class 2A, member 1
• Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Alpha-mannosidase II Lysate (NBL1-12834). (Showing 1 - 1 of 1 FAQ).

1. How much lysate is used in WB example? How much is recommended for positive control?
   • The Western blot image you see shows 5ug of protein. 5-10ug of lysate may be used depending on the sensitivity of the antibody.

Bioinformatics

• Gene Symbol: MAN2A1