| Reactivity | HuSpecies Glossary |
| Applications | IHC, IHC-P |
| Clone | C2 + C3 + MBS-12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | HRP |
| Immunogen | Recombinant full-length human Alpha fetoprotein |
| Localization | Cytoplasmic |
| Marker | Hepatocellular/Germ Cell Tumor Marker |
| Specificity | It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AFP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-48013AF350 | |
| Alexa Fluor 405 | NBP2-48013AF405 | |
| Alexa Fluor 488 | NBP2-48013AF488 | |
| Alexa Fluor 532 | NBP2-48013AF532 | |
| Alexa Fluor 594 | NBP2-48013AF594 | |
| Alexa Fluor 647 | NBP2-48013AF647 | |
| Alexa Fluor 700 | NBP2-48013AF700 | |
| Alexa Fluor 750 | NBP2-48013AF750 | |
| Allophycocyanin | NBP2-48013APC | |
| Biotin | NBP2-48013B | |
| DyLight 350 | NBP2-48013UV | |
| DyLight 405 | NBP2-48013V | |
| DyLight 488 | NBP2-48013G | |
| DyLight 550 | NBP2-48013R | |
| DyLight 594 | NBP2-48013DL594 | |
| DyLight 650 | NBP2-48013C | |
| DyLight 680 | NBP2-48013FR | |
| DyLight 755 | NBP2-48013IR | |
| FITC | NBP2-48013F | |
| HRP | NBP2-48013H | |
| Janelia Fluor 549 | NBP2-48013JF549 | |
| Janelia Fluor 646 | NBP2-48013JF646 | |
| PE | NBP2-48013PE | |
| PerCP | NBP2-48013PCP |
Diseases for alpha-Fetoprotein/AFP Antibody (NBP2-48013H)Discover more about diseases related to alpha-Fetoprotein/AFP Antibody (NBP2-48013H).
| Pathways for alpha-Fetoprotein/AFP Antibody (NBP2-48013H)View related products by pathway.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | AFP |
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/images2/CEACAM5-CD66e-Antibody-(CEA31)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0003.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/images2/CEACAM5-CD66e-Antibody-(CEA31)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0001.jpg)
![Immunohistochemistry-Paraffin: CEACAM5/CD66e Antibody (CEA31) - Azide and BSA Free [NBP2-34594] - Human Colon Carcinoma stained with CEACAM5/CD66e Antibody (CEA31).](http://images.novusbio.com/images2/CEACAM5-CD66e-Antibody-(CEA31)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34594-img0002.jpg)
![SDS-Page: Recombinant Human PSG2 Protein [H00005670-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00005670-Q01-1.jpg)
![Western Blot: FAM126A Antibody [NBP1-32914] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/images2/FAM126A Antibody-Western Blot-NBP1-32914-img0004.jpg)
![Western Blot: FAM126A Antibody [NBP1-32914] - Sample (50 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/images2/FAM126A Antibody-Western Blot-NBP1-32914-img0003.jpg)
![Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Western-Blot-NBP1-58268-img0007.jpg)
![Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-NBP1-58268-img0008.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-Paraffin-NBP1-58268-img0005.jpg)
![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/images2/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Staining of HepG2 (A) and Daudi cell lysates with antibody at 1 ug/mL (B) and HeLa (C) and Jurkat (D) cell lysates with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/images2/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NB100-1519-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic and nuclear/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/images2/Acetylcholinesterase-ACHE-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1519-img0002.jpg)
![Flow Cytometry: Acetylcholinesterase/ACHE Antibody [NB100-1519] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/Acetylcholinesterase-ACHE-Antibody-Flow-Cytometry-NB100-1519-img0003.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Analysis using Anti-SERPINA1 antibody NBP1-90309 (A) shows similar pattern to independent antibody NBP1-90308 (B).](http://images.novusbio.com/images2/Serpin-A1-alpha-1-Antitrypsin-Antibody-Western-Blot-NBP1-90309-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human cell line Hep G2 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90309-img0009.jpg)
![Immunohistochemistry-Paraffin: Serpin A1/alpha 1-Antitrypsin Antibody [NBP1-90309] - Staining of human placenta shows strong positivity in plasma in blood vessels.](http://images.novusbio.com/images2/Serpin-A1-alpha-1-Antitrypsin-Antibody-Immunohistochemistry-Paraffin-NBP1-90309-img0028.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/images2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vimentin Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0008.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0007.jpg)