alpha COP I Antibody

Immunocytochemistry/Immunofluorescence: alpha COP I Antibody [NB120-2913] - HeLa cells.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

alpha COP I Antibody Summary

• Description: Novus Biologicals Rabbit alpha COP I Antibody (NB120-2913) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.
• Localization: Cytoplasmic and Golgi Apparatus
• Specificity: Detects coatomer-protein I alpha (COP I alpha).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: COPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 3 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: WB: Detects an approx. 140 kDa protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at approx. 100 kDa. ICC: Staining of alpha COP I results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm.
• Theoretical MW: 138 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for alpha COP I Antibody

• alpha coat protein
• Alpha-coat protein
• Alpha-COP
• coatomer protein complex, subunit alpha
• coatomer subunit alpha
• FLJ26320
• HEPCOP
• HEP-COPalpha-COP
• xenin

Background

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COPA
• Entrez:
  • 1314(Human)
  • 304978(Rat)
• Uniprot:
  • G3V6T1(Rat)