ALOXE3 Antibody

Western Blot: ALOXE3 Antibody [NBP1-32533] - Sample(30 ug whole cell lysate)A:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: ALOXE3 Antibody [NBP1-32533] - NCI-N87 xenograft, using ALOXE3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ALOXE3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ALOXE3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALOXE3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ALOXE3 Antibody

• arachidonate lipoxygenase 3
• EC 1.13.11.-
• E-LOX
• eLOX3
• e-LOX-3
• epidermal lipoxygenase
• epidermis-type lipoxygenase 3
• MGC119694
• MGC119695
• MGC119696

Background

The leukotrienes constitute a group of arachidonic acid-derived compounds with biologic activities suggesting important roles in inflammation and immediate hypersensitivity. These compounds are catabolized, in part, by lipoxygenases. Epidermis-type lipoxygenases, a distinct subclass within the multigene family of mammalian lipoxygenases, are novel isoenzymes isolated from human and mouse skin including human ALOX15B (MIM 603697), human and mouse ALOX12B (MIM 603741), mouse 8S-LOX, and mouse e-LOX-3.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ALOXE3 Antibody (NBP1-32533)(2)

Publications using NBP1-32533

• Yang X, Liu J, Wang C et al. miR-18a promotes glioblastoma development by down-regulating ALOXE3-mediated ferroptotic and anti-migration activities Oncogenesis 2021-02-12 [PMID: 33579899] (IHC, Human)
• Sasaki K, Akiyama M, Yanagi T et al. CYP4F22 is highly expressed at the site and timing of onset of keratinization during skin development. J Dermatol Sci. 2011-12-13 [PMID: 22209317]

Bioinformatics

• Gene Symbol: ALOXE3