ALMS1 Antibody

Immunocytochemistry/Immunofluorescence: ALMS1 Antibody [NB100-1195] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: ALMS1 Antibody [NB100-1195] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ALMS1 Antibody Summary

• Immunogen: Peptide with sequence C-RVTNQLLGRKVPWD corresponding to C-Terminus according to NP_055935.4.
• Epitope: RVTNQLLGRKVPWD
• Specificity: The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALMS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
• Control Peptide:

  ALMS1 Peptide (NB100-1195PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALMS1 Antibody

• Alstrom syndrome 1
• Alstrom syndrome protein 1
• DKFZp686A118
• DKFZp686D1828
• KIAA0328ALSS

Background

This protein contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALMS1 Antibody (NB100-1195)(1)

Publication using NB100-1195

• Collin GB, Marshall JD, Ikeda A et al. Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in Alstrom syndrome. Nat Genet 2002-05-01 [PMID: 11941369]

Bioinformatics

• Gene Symbol: ALMS1
• Entrez:
  • 7840(Human)
  • 236266(Mouse)
  • 297408(Rat)
• Uniprot:
  • NP_055935(Human)