ALKBH5 Antibody

Images

 
Western Blot: ALKBH5 Antibody [NBP1-82188] - ZNF217 and ALKBH5 regulate NANOG and KLF4 expression via modulation of m6A levels. MCF-7 subclones were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. ...read more
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Immunohistochemical analysis of human breast cancer biopsies. Immunohistochemistry was performed on breast cancer biopsies using ALKBH5 and HIF-1a ...read more
Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis of ALKBH5 in MDA-MB-231 human breast cancer subclones using anti-ALKBH5 antibody. Image from verified customer review.
Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human liver shows weak to moderate nuclear positivity in hepatocytes.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human kidney shows nuclear positivity.
Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ALKBH5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVD
Specificity
Specificity of human ALKBH5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALKBH5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
WB, IHC-P reported in scientific literature (PMID: 27590511). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ALKBH5 antibody validated for WB from a verified customer review.
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ALKBH5 Recombinant Protein Antigen (NBP1-82188PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-82188 in the following applications:

Publications
Read Publications using
NBP1-82188 in the following applications:

  • IHC
    2 publications
  • 3 publications
  • WB
    4 publications

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32329191).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ALKBH5 Antibody

  • ABH5OFOXD
  • alkB, alkylation repair homolog 5 (E. coli)
  • Alkylated DNA repair protein alkB homolog 5
  • EC 1.14.11.-
  • FLJ20308
  • OFOXD1
  • oxoglutarate and iron-dependent oxygenase domain containing
  • probable alpha-ketoglutarate-dependent dioxygenase ABH5

Background

ALKBH5, RNA demethylase ALKBH5, ALKB homolog 5 (44 kDa) is a dioxygenase enzyme which demethylates RNA in a reaction requiring oxygen, alpha-ketoglutarate, and iron. ALKBH5 and fat mass and obesity-associated protein (FTO) are involved in the demethylation of N6-methyladenosine or m6A mRNA, the most common post-translational modification in eukaryotic mRNA. m6A mRNA modifications are very dynamic; they are introduced into the mRNA by m6A methyltransferases (e.g., METTL3/14 and KIAA1429), removed by erasers such as FTO and ALKBH5, and decoded by m6A binding proteins (e.g., IGF2BP1 and YTHDF1/2/3). m6A mRNA modifications play roles in regulating mRNA splicing, processing, translation and stability. m6A mRNA modifications are also associated with pathological states including cancer (1, 2).

ALKBH5 has been implicated in various physiological and pathophysiological processes. In mice, ALKBH5 is highly expressed in the testes where loss of ALKBH5 expression leads to decreased spermatogenesis, abnormal spermatozoa and increased apoptosis (3). In glioblastoma, ALKBH5 overexpression promotes stemness and tumor progression (2). In breast cancer cells under hypoxic conditions, HIF-1 alpha and HIF-2 alpha induce the expression of ALKH5 and leads to increased demethylation and expression of Nanog thus promoting breast cancer stem cell properties (4).

References

1. Chen, X. Y., Zhang, J., & Zhu, J. S. (2019). The role of m6A RNA methylation in human cancer. Molecular Cancer. https://doi.org/10.1186/s12943-019-1033-z

2. Deng, X., Su, R., Weng, H., Huang, H., Li, Z., & Chen, J. (2018). RNA N 6 -methyladenosine modification in cancers: Current status and perspectives. Cell Research. https://doi.org/10.1038/s41422-018-0034-6

3. Meyer, K. D., & Jaffrey, S. R. (2014). The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm3785

4. Zhang, C., Samanta, D., Lu, H., Bullen, J. W., Zhang, H., Chen, I.,... Semenza, G. L. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1602883113

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for ALKBH5 Antibody (NBP1-82188)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(3)
WB
(4)
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Filter By Species
Human
(4)
Mouse
(1)
All Species

Review for ALKBH5 Antibody (NBP1-82188) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-82188:
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WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ALKBH5 NBP1-82188
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5
reviewed by:
Anonymous
WB Human 05/29/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
05/29/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ALKBH5
Entrez
Uniprot