ALKBH5 Antibody

Western Blot: ALKBH5 Antibody [NBP1-82188] - ZNF217 and ALKBH5 regulate NANOG and KLF4 expression via modulation of m6A levels. MCF-7 subclones were exposed to 20% or 1% O2 for 48 h and immunoblot assays were performed. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/11743/text/), licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Immunohistochemical analysis of human breast cancer biopsies. Immunohistochemistry was performed on breast cancer biopsies using ALKBH5 and HIF-1a antibodies. Representative negative and positive staining (biopsies from patients #2 and #3, respectively) are shown. Scale bar: 500 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/11743/text/), licensed under a CC-BY licence.

Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis of ALKBH5 in MDA-MB-231 human breast cancer subclones using anti-ALKBH5 antibody. Image from verified customer review.

Western Blot: ALKBH5 Antibody [NBP1-82188] - Analysis in human cell line U-87 MG.

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human liver shows weak to moderate nuclear positivity in hepatocytes.

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human kidney shows nuclear positivity.

Immunohistochemistry-Paraffin: ALKBH5 Antibody [NBP1-82188] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ALKBH5 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EKLKSMTSRDNYKAGSREAAAAAAAAVAAAAAAAAAAEPYPVSGAKRKYQEDSDPERSDYEEQQLQKEEEARKVKSGIRQMRLFSQDECAKIEARIDEVVSRAEKGLYNEHTVD
• Specificity: Specificity of human ALKBH5 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
• Predicted Species: Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALKBH5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: WB, IHC-P reported in scientific literature (PMID: 27590511). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ALKBH5 antibody validated for WB from a verified customer review.
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ALKBH5 Recombinant Protein Antigen (NBP1-82188PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32329191).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ALKBH5 Antibody

• ABH5OFOXD
• alkB, alkylation repair homolog 5 (E. coli)
• Alkylated DNA repair protein alkB homolog 5
• EC 1.14.11.-
• FLJ20308
• OFOXD1
• oxoglutarate and iron-dependent oxygenase domain containing
• probable alpha-ketoglutarate-dependent dioxygenase ABH5

Background

ALKBH5, RNA demethylase ALKBH5, ALKB homolog 5 (44 kDa) is a dioxygenase enzyme which demethylates RNA in a reaction requiring oxygen, alpha-ketoglutarate, and iron. ALKBH5 and fat mass and obesity-associated protein (FTO) are involved in the demethylation of N6-methyladenosine or m6A mRNA, the most common post-translational modification in eukaryotic mRNA. m6A mRNA modifications are very dynamic; they are introduced into the mRNA by m6A methyltransferases (e.g., METTL3/14 and KIAA1429), removed by erasers such as FTO and ALKBH5, and decoded by m6A binding proteins (e.g., IGF2BP1 and YTHDF1/2/3). m6A mRNA modifications play roles in regulating mRNA splicing, processing, translation and stability. m6A mRNA modifications are also associated with pathological states including cancer (1, 2).

ALKBH5 has been implicated in various physiological and pathophysiological processes. In mice, ALKBH5 is highly expressed in the testes where loss of ALKBH5 expression leads to decreased spermatogenesis, abnormal spermatozoa and increased apoptosis (3). In glioblastoma, ALKBH5 overexpression promotes stemness and tumor progression (2). In breast cancer cells under hypoxic conditions, HIF-1 alpha and HIF-2 alpha induce the expression of ALKH5 and leads to increased demethylation and expression of Nanog thus promoting breast cancer stem cell properties (4).

References

1. Chen, X. Y., Zhang, J., & Zhu, J. S. (2019). The role of m6A RNA methylation in human cancer. Molecular Cancer. https://doi.org/10.1186/s12943-019-1033-z

2. Deng, X., Su, R., Weng, H., Huang, H., Li, Z., & Chen, J. (2018). RNA N 6 -methyladenosine modification in cancers: Current status and perspectives. Cell Research. https://doi.org/10.1038/s41422-018-0034-6

3. Meyer, K. D., & Jaffrey, S. R. (2014). The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/nrm3785

4. Zhang, C., Samanta, D., Lu, H., Bullen, J. W., Zhang, H., Chen, I.,... Semenza, G. L. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America. https://doi.org/10.1073/pnas.1602883113

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALKBH5 Antibody (NBP1-82188)(5)

Publications using NBP1-82188

• Querido E, Sfeir A, Chartrand P ALKBH5 inhibited cell proliferation and sensitized bladder cancer cells to cisplatin by m6A-CK2 alpha mediated glycolysis STAR Protoc Oct 1 2020 [PMID: 33111129] (IHC-P, WB, Human)
• Jiang Y, Wan Y, Gong M et al. RNA demethylase ALKBH5 promotes ovarian carcinogenesis in a simulated tumour microenvironment through stimulating NF-kappa B pathway J. Cell. Mol. Med. Apr 24 2020 [PMID: 32329191] (IHC, IHC-P, Mouse)
• The m6A Demethylase ALKBH5 Controls Trophoblast Invasion at the Maternal-Fetal Interface by Regulating the Stability of CYR61 mRNA Li X, Jin F, Wang B et al. Theranostics. [PMID: 31281518] (WB, IHC, Human)
• Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016 Aug 31 [PMID: 27590511] (WB, IHC-P, Human)
• Zhang C, Samanta D, Lu H et al. Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proc Natl Acad Sci U S A. 2016 Mar 21 [PMID: 27001847] (WB, Human)

Review for ALKBH5 Antibody (NBP1-82188) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 05/29/2015

Summary

• Application: Western Blot
• Species: Human
• File: View PDF

Bioinformatics

• Gene Symbol: ALKBH5
• Entrez:
  • 54890(Human)
• Uniprot:
  • Q6P6C2(Human)