Alkaline Phosphatase, Tissue Non-Specific Antibody

Images

 
Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Sh, Rt, Fe, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.48 mg/ml

Order Details

Alkaline Phosphatase, Tissue Non-Specific Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Alkaline Phosphatase (Tissue Non-Specific). The exact sequence is proprietary.
Localization
Cell membrane, Lipid-anchor, GPI-anchor
Predicted Species
Rat (91%), Rhesus Macaque (97%), Feline (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ALPL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (90%), Bovine (89%), Dog (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.48 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody

  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALPMGC167935

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ALPL