Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0)

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunohistochemistry of Alkaline Phosphatase, Tissue Non-Specific in paraffin-embedded Human lung cancer tissue using NBP3-19565 at dilution 1/50

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S08-6H0
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Alkaline Phosphatase, Tissue Non-Specific (Uniprot # P05186)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALPL
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/100
  • Immunohistochemistry 1/50-1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0)

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPL