Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Ca
• Applications: WB
• Clone: HL2003
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free (NBP3-25356) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of Alkaline Phosphatase, Tissue Non-Specific.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALPL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free

• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALPMGC167935

Background

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALPL