Alkaline Phosphatase/ALPP Overexpression Lysate

Images

 
Western Blot: Alkaline Phosphatase/ALPP Overexpression Lysate [NBL1-07488] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Alkaline Phosphatase, Placental.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Alkaline Phosphatase/ALPP Overexpression Lysate Summary

Description

Alkaline Phosphatase/ALPP Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210504

Accession#: NM_001632

Protein Tag: C-MYC/DDK
Gene
ALPP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
55.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Alkaline Phosphatase/ALPP Overexpression Lysate

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC 3.1.3.1
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-20383
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
355-BM/CF
Species: Hu, Mu, Rt
Applications: BA
MAB7665
Species: Hu
Applications: IHC, WB
H00002678-M01
Species: Hu, Mu, Ye
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA, WB
AF808
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP2-54724
Species: Hu
Applications: IHC, IHC-P, WB
AF3398
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
AF4014
Species: Hu
Applications: WB
355-BM/CF
Species: Hu, Mu, Rt
Applications: BA
H00138428-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-89953
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
DY805
Species: Hu
Applications: ELISA
NB300-164
Species: Ca, Hu, Mu, Po, Pm, Rt(-)
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
210-TA
Species: Hu
Applications: BA
MAB7547
Species: Hu
Applications: ICC, WB

Publications for Alkaline Phosphatase/ALPP Lysate (NBL1-07488) (0)

There are no publications for Alkaline Phosphatase/ALPP Lysate (NBL1-07488).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Alkaline Phosphatase/ALPP Lysate (NBL1-07488) (0)

There are no reviews for Alkaline Phosphatase/ALPP Lysate (NBL1-07488). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Alkaline Phosphatase/ALPP Lysate (NBL1-07488). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Additional Alkaline Phosphatase/ALPP Products

Bioinformatics Tool for Alkaline Phosphatase/ALPP Lysate (NBL1-07488)

Discover related pathways, diseases and genes to Alkaline Phosphatase/ALPP Lysate (NBL1-07488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Alkaline Phosphatase/ALPP Lysate (NBL1-07488)

Discover more about diseases related to Alkaline Phosphatase/ALPP Lysate (NBL1-07488).
 

Pathways for Alkaline Phosphatase/ALPP Lysate (NBL1-07488)

View related products by pathway.

PTMs for Alkaline Phosphatase/ALPP Lysate (NBL1-07488)

Learn more about PTMs related to Alkaline Phosphatase/ALPP Lysate (NBL1-07488).
 

Research Areas for Alkaline Phosphatase/ALPP Lysate (NBL1-07488)

Find related products by research area.

Blogs on Alkaline Phosphatase/ALPP

There are no specific blogs for Alkaline Phosphatase/ALPP, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Alkaline Phosphatase/ALPP Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ALPP