Alkaline Phosphatase/ALPP Antibody (H7E8)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clone
H7E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Alkaline Phosphatase/ALPP Antibody (H7E8) Summary

Immunogen
Purified placental alkaline phosphatase.
Localization
Attached to the membrane by a GPI-anchor.
Specificity
The antibody H7E8 recognizes throphoblast-derived membrane-associated enzyme alkaline phosphatase.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ALPP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Predicted molecular weight: 58 kDa.
Positive Control
Alkaline Phosphatase/ALPP Lysate (NBP2-64909)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Alkaline Phosphatase/ALPP Antibody (H7E8)

  • Alkaline phosphatase Regan isozyme
  • Alkaline Phosphatase
  • alkaline phosphatase, placental (Regan isozyme)
  • alkaline phosphatase, placental type
  • alkaline phosphatase, placental
  • alkaline phosphomonoesterase
  • ALP
  • ALPP
  • EC 3.1.3.1
  • FLJ61142
  • glycerophosphatase
  • PALP
  • Placental alkaline phosphatase 1
  • PLAP
  • PLAP-1
  • Regan isozyme
  • SEAP

Background

Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Alkaline Phosphatase/ALPP Antibody (NB500-532). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
    • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALPP
Entrez