Alkaline Phosphatase/ALPP Antibody (GM022)

Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (GM022) [NBP2-44958] - Human Placenta stained with PLAP Monoclonal Antibody (GM022).

• Reactivity: Hu
• Applications: Flow, IHC, IHC-P, ICC/IF
• Clone: GM022
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Alkaline Phosphatase/ALPP Antibody (GM022) Summary

• Immunogen: Recombinant human PLAP protein
• Localization: Cytoplasmic and cell surface
• Marker: Germ Cell Tumor Marker
• Specificity: Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 2q37.1
  Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Alkaline Phosphatase/ALPP Antibody (GM022)

• Alkaline phosphatase Regan isozyme
• Alkaline Phosphatase
• alkaline phosphatase, placental (Regan isozyme)
• alkaline phosphatase, placental type
• alkaline phosphatase, placental
• alkaline phosphomonoesterase
• ALP
• ALPP
• EC 3.1.3.1
• FLJ61142
• glycerophosphatase
• PALP
• Placental alkaline phosphatase 1
• PLAP
• PLAP-1
• Regan isozyme
• SEAP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Alkaline Phosphatase/ALPP Antibody (NBP2-44958). (Showing 1 - 1 of 1 FAQ).

1. I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
   • None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.

Bioinformatics

• Gene Symbol: ALPP
• Uniprot:
  • P05187()