Alkaline Phosphatase/ALPP Antibody (8B6) [FITC] Summary
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Cell membrane; Lipid-anchor > GPI-anchor
Alkaline Phosphatase, Placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of Alkaline Phosphatase.
Protein A purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Packaging, Storage & Formulations
Store at 4C in the dark.
FITC (A=495, E=519)
0.05% Sodium Azide
Protein A purified
- Western Blot 1:1000
- ELISA 1:100-1:2000
- Flow Cytometry
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
This Alkaline Phosphatase, Placental Antibody (8B6) is useful for Immunohistochemistry on paraffin-embedded sections and frozen sections, Immunocytochemistry/Immunofluorescence, ELISA, Western blot and Radioimmunodiffusion. In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. Use in Flow Cytometry reported in various pieces of scientific literature
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human. Not yet tested in other species.
Alternate Names for Alkaline Phosphatase/ALPP Antibody (8B6) [FITC]
- Alkaline phosphatase Regan isozyme
- Alkaline Phosphatase
- alkaline phosphatase, placental (Regan isozyme)
- alkaline phosphatase, placental type
- alkaline phosphatase, placental
- alkaline phosphomonoesterase
- EC 22.214.171.124
- Placental alkaline phosphatase 1
- Regan isozyme
Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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FAQs for Alkaline Phosphatase/ALPP Antibody (NB110-3638F). (Showing 1 - 1 of 1 FAQ).
I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
- None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
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