Alkaline Phosphatase/ALPL Antibody (B4-78) [Biotin]

Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, IHC, ICC/IF
• Clone: B4-78
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

Alkaline Phosphatase/ALPL Antibody (B4-78) [Biotin] Summary

• Immunogen: Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
• Specificity: Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (B4-78) [Biotin]

• Akp2
• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• ALPL
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALP
• TNSALPMGC167935

Background

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase/ALPL Antibody (BAM1448)(4)

Publications using BAM1448

• MM Weivoda, CK Chew, DG Monroe, JN Farr, EJ Atkinson, JR Geske, B Eckhardt, B Thicke, M Ruan, AJ Tweed, LK McCready, RA Rizza, A Matveyenko, M Kassem, TL Andersen, A Vella, MT Drake, BL Clarke, MJ Oursler, S Khosla Identification of osteoclast-osteoblast coupling factors in humans reveals links between bone and energy metabolism Nat Commun, 2020-01-07;11(1):87. 2020-01-07 [PMID: 31911667] (MACS, Human)
• T Kuroiwa, M Matsumoto, R Kato, A Nimura, T Yoshii, A Okawa, K Fujita Activation of cancer-related and mitogen-activated protein kinase signaling pathways in human mature osteoblasts isolated from patients with type 2 diabetes Bone Rep, 2019-02-21;10(0):100199. 2019-02-21 [PMID: 30891471] (Cell Selection, Human)
• Drake MT, Srinivasan B, Modder UI, Ng AC, Undale AH, Roforth MM, Peterson JM, McCready LK, Riggs BL, Khosla S Effects of intermittent parathyroid hormone treatment on osteoprogenitor cells in postmenopausal women. Bone, 2011-05-11;49(3):349-55. 2011-05-11 [PMID: 21600325] (ICC, Human)
• Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering. Biomaterials, 2009-04-10;30(20):3415-27. 2009-04-10 [PMID: 19362364] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ALPL