Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

Images

 
BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open ...read more
Alkaline Phosphatase/ALPL and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct‑3/4 ...read more
Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ICC/IF
Clone
B4-78
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated] Summary

Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALPL
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB1448 in the following applications:

Publications
Read Publications using
MAB1448 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

  • Akp2
  • Alkaline phosphatase liver/bone/kidney isozyme
  • alkaline phosphatase, liver/bone/kidney
  • alkaline phosphatase, tissue-nonspecific isozyme
  • alkaline phosphomonoesterase
  • ALPL
  • APTNAP
  • AP-TNAP
  • EC 3.1.3.1
  • FLJ40094
  • FLJ93059
  • glycerophosphatase
  • HOPS
  • liver/bone/kidney-type alkaline phosphatase
  • MGC161443
  • tissue-nonspecific ALP
  • TNAP
  • TNSALP
  • TNSALPMGC167935

Background

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Alkaline Phosphatase/ALPL Antibody (MAB1448)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Flow, ICC, IHC Paraffin-embedded, WB.


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(1)
ICC
(4)
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(1)
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(2)
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(3)
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Showing Publications 1 - 7 of 7.
Publications using MAB1448 Applications Species
Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS ONE, 2015;10(3):e0118499. 2015 [PMID: 25764185] (ICC,, Human) ICC Human
Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy. J Immunol Res, 2014;2014(0):230346. 2014 [PMID: 24877156] (Flow,, Human) Flow Human
Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells. Bone, 2011;48(6):1417-26. 2011 [PMID: 21376148] (IHC Paraffin-embedded,, Rat) IHC Paraffin-embedded Rat
Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro. Bone, 2009;45(2):309-20. 2009 [PMID: 19379851] (ICC,, Mouse) ICC Mouse
Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development. Dev. Biol., 2009;325(1):129-37. 2009 [PMID: 18996110] (WB,, Mouse) WB Mouse
Valarmathi MT, Yost MJ, Goodwin RL, Potts JD The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold. Biomaterials, 2008;29(14):2203-16. 2008 [PMID: 18289664] (ICC,, Rat) ICC Rat
Otsuru S, Tamai K, Yamazaki T, Yoshikawa H, Kaneda Y Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice. Biochem. Biophys. Res. Commun., 2007;354(2):453-8. 2007 [PMID: 17239347] (ICC,, Mouse) ICC Mouse

Review for Alkaline Phosphatase/ALPL Antibody (MAB1448) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1448:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Human 02/10/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 23514783
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23514783

Details

Detection NotesSee PMID 23514783

Comments

CommentsIndependent review from antibodyresource.comThe antibody was used for assessment of osteogenic differentiation of mesenchymal stem cells. Surface expression of ALP determined by the antibody correlated well with ALP activity.

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Recent Reviews

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Anonymous
02/10/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol ALPL
Entrez