Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

Alkaline Phosphatase/ALPL and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready, ICC/IF
• Clone: B4-78
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated] Summary

• Immunogen: Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
• Specificity: Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
• Details of Functionality: ActivityAssaywCitation not found None None None
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALPL

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

• Akp2
• Alkaline phosphatase liver/bone/kidney isozyme
• alkaline phosphatase, liver/bone/kidney
• alkaline phosphatase, tissue-nonspecific isozyme
• alkaline phosphomonoesterase
• ALPL
• APTNAP
• AP-TNAP
• EC 3.1.3.1
• FLJ40094
• FLJ93059
• glycerophosphatase
• HOPS
• liver/bone/kidney-type alkaline phosphatase
• MGC161443
• tissue-nonspecific ALP
• TNAP
• TNSALP
• TNSALPMGC167935

Background

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Alkaline Phosphatase/ALPL Antibody (MAB1448)(8)

Publications using MAB1448

• J Camps, H Grosemans, R Gijsbers, C Maes, M Sampaolesi Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells Int J Mol Sci, 2019;20(5):. 2019 [PMID: 30841538] (Flow Cytometry, Human)
• Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits. PLoS ONE, 2015;10(3):e0118499. 2015 [PMID: 25764185] (ICC, Human)
• Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy. J Immunol Res, 2014;2014(0):230346. 2014 [PMID: 24877156] (Flow Cytometry, Human)
• Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells. Bone, 2011;48(6):1417-26. 2011 [PMID: 21376148] (IHC-P, Rat)
• Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro. Bone, 2009;45(2):309-20. 2009 [PMID: 19379851] (ICC, Mouse)
• Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development. Dev. Biol., 2009;325(1):129-37. 2009 [PMID: 18996110] (Western Blot, Mouse)
• Valarmathi MT, Yost MJ, Goodwin RL, Potts JD The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold. Biomaterials, 2008;29(14):2203-16. 2008 [PMID: 18289664] (ICC, Rat)
• Otsuru S, Tamai K, Yamazaki T, Yoshikawa H, Kaneda Y Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice. Biochem. Biophys. Res. Commun., 2007;354(2):453-8. 2007 [PMID: 17239347] (ICC, Mouse)

Review for Alkaline Phosphatase/ALPL Antibody (MAB1448) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous Flow Human 02/10/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 23514783
• Species: Human

Blocking

• Blocking Details: See PMID 23514783

Details

• Detection Notes: See PMID 23514783

Comments

• Comments: Independent review from antibodyresource.comThe antibody was used for assessment of osteogenic differentiation of mesenchymal stem cells. Surface expression of ALP determined by the antibody correlated well with ALP activity.

Bioinformatics

• Gene Symbol: ALPL