Alkaline Phosphatase/ALPL Antibodies: Novus Biologicals

AF2910

Alkaline Phosphatase/ALPL Antibody [Unconjugated]

100 μg

AF2910

HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue).<EM>Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA.</EM>View our protocol for <a class=NoLineLink href=

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Downregulation of HDAC9 rescued lineage differentiation imbalance and ameliorated senescence in aged BMMSCs. a Alizarin Red staining was performed, and osteogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. b Oil Red O staining was performed, and adipogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. c Alizarin Red staining was performed, and osteogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. d Oil Red O staining was performed, and adipogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. e, f Expressions of the senescence-related proteins p53 and p-p53 in BMMSCs cultured in vitro from aged mice (e) and young mice (f) were examined by western blotting. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of HDAC9 improved the lineage differentiation of endogenous BMMSCs ex vivo. The BMMSCs harvested from the mice from the control, shScr-treated, & shHDAC9-treated groups 4 weeks after bone intrainjection. e Alizarin Red staining was performed, & osteogenesis-related proteins detected in BMMSCs from the three group. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

The representative images of Ki‐67 staining (A) and quantified by the positive‐stained percentage through ImageJ software (B). Scale bar, 100 μm. (C‐D) Alizarin Red staining was performed to detect mineralized nodules formed in Con, AS‐Exo and NS‐Exo 14 days after osteogenic induction and quantified with a spectrophotometer after dissolving with cetylpyridinium chloride. (E‐F) Lipid droplet formation was detected by Oil Red O staining 7 days after adipogenic induction with positive area quantified. Scale bar, 100 μm. (G‐H) The protein expression levels of Runx2, ALP and PPAR‐ gamma in Con, AS‐Exo and NS‐Exo groups were measured through Western blot and quantified by ImageJ software. n = 3 per group. Data are shown as mean ± SD; ns, not significant; *P < .05; **P < .01; ***P < .001. AS‐Exo, adult serum exosomes; Con, control; NS‐Exo, neonatal serum exosomes Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32608556), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of autophagy blocked the ability of HDAC9 siRNA to rebalance BMMSC differentiation. To investigate the HDAC9-autophagy axis regulating BMMSC function, BMMSCs were respectively transfected with Nc siRNA and HDAC9 siRNA or co-transfected with HDAC9 siRNA and BECN1 siRNA. a Alizarin Red staining was performed, and osteogenesis-related proteins were analyzed in aged BMMSCs from above three groups. b Oil Red O staining was performed, and adipogenesis-related proteins were analyzed in three groups of cells described above. Scale bars = 100 μm. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2910U-100UG
Alexa Fluor 405	AF2910V-100UG
Alexa Fluor 488	AF2910G-100UG
Alexa Fluor 532	AF2910X-100UG
Alexa Fluor 594	AF2910T-100UG
Alexa Fluor 647	AF2910R-100UG
Alexa Fluor 700	AF2910N-100UG
Alexa Fluor 750	AF2910S-100UG
Alexa Fluor Plus 405	AF2910AFP405-100UG
Alexa Fluor Plus 488	AF2910AFP488-100UG
Alexa Fluor Plus 555	AF2910AFP555-100UG
Alexa Fluor Plus 594	AF2910AFP594-100UG
Alexa Fluor Plus 647	AF2910AFP647-100UG
Alexa Fluor Plus 680	AF2910AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2910-SP

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MAB1448

Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

(1 Review)

(15 Publications)

100 μg

MAB1448

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." class="big_lightbox">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." alt="BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." class="big_thumb" />

AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Alkaline Phosphatase/ALPL and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (<a class=

AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Whole blood granulocytes were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Immunocytochemistry of pluripotency markers.Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc immortalized lines (hNS1, hVM1, and hCTX) and fibroblasts (hFF-1). From top to bottom the markers studied were: NANOG, OCT3/4, SOX2 and AP. Hoechst 33258 nuclear staining is shown in blue. Scale bar represents 100 μm for hESCs in the left hand column (low magnification) and 25 μm for all the other microphotographs. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0118499), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1448U-100UG
Alexa Fluor 405	FAB1448V-100UG
Alexa Fluor 488	FAB1448G-100UG
Alexa Fluor 594	FAB1448T-100UG
Alexa Fluor 647	FAB1448R-100UG
Alexa Fluor 700	FAB1448N-100UG
Alexa Fluor 750	FAB1448S-100UG
Alexa Fluor Plus 405	FAB1448AFP405-100UG
Alexa Fluor Plus 488	FAB1448AFP488-100UG
Alexa Fluor Plus 555	FAB1448AFP555-100UG
Alexa Fluor Plus 594	FAB1448AFP594-100UG
Alexa Fluor Plus 647	FAB1448AFP647-100UG
Alexa Fluor Plus 680	FAB1448AFP680-100UG
Allophycocyanin	FAB1448A-025
Allophycocyanin	FAB1448A-100
Biotin	BAM1448
Phycoerythrin	FAB1448P-025
Phycoerythrin	FAB1448P-100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1448-SP
Carrier Free	FAB1448P-100
Carrier Free	FAB1448P-025
Carrier Free	FAB1448A-100
Carrier Free	FAB1448A-025
Carrier Free	FAB1448V-100UG
Carrier Free	FAB1448U-100UG
Carrier Free	FAB1448T-100UG
Carrier Free	FAB1448S-100UG
Carrier Free	FAB1448R-100UG
Carrier Free	FAB1448N-100UG
Carrier Free	FAB1448G-100UG

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MAB29092

Alkaline Phosphatase/ALPL Antibody (928929) [Unconjugated]

(5 Publications)

100 μg

MAB29092

HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, Saos‑2 human osteosarcoma cells and BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for Alkaline Phosphatase/ALPL at approximately 115 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) . This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Analysis of the control and CAVD aortic valve samples (n = 5 and 5, respectively). (A) Western blotting for CDK1 and ALP proteins on valve tissues. (B) Semi-quantitative analysis of protein expression. (C) Immunofluorescence staining of CDK1 and Ki-67 on valve tissues. (D) Semi-quantitative analysis of fluorescence intensity. (E) PCR test for cell proliferation genes on valve tissues. (*) p < 0.05 indicates a significant difference. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35571082), licensed under a CC-BY license. Not internally tested by R&D Systems.

CCND1 participates in the osteogenic differentiation of VICs following OM. A–F) VICs were transfected with CCND1 siRNA or scrambled siRNA, and then stimulated with OM for 7 days. Immunoblot analysis of CCND1, ALP, Runx2 P53, and P21 expression in VICs from indicated groups (n = 3, each group). Bar plots showing the semiquantitative analysis of indicated genes expression. G,H) With OM induction for 7 days, representative ALP staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. I–K) With OM induction for 21 days, representative Alizarin red staining showed the calcific nodules in VICs from indicated groups (n = 3, each group). Scale bar 50 µm. L–M) Representative SA‐ beta ‐gal staining of VICs from indicated groups (n = 3, each group). Bar plot showing the percentage of SA‐ beta ‐gal staining positive cells. Scale bar 50 µm. Data are means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Morusin attenuates VIC calcification by activating Nrf2 signaling pathway. A–D) ML385 was used to inhibit the activation of Nrf2 in VICs. Representative immunoblot images and quantification of the levels of ALP, Runx2, and P21 in VICs from indicated groups (n = 3, each group). E,F) With OM induction for 7 days, representative ALP staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. G–I) With OM induction for 21 days, representative Alizarin red staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. Data are means ± SD. **p < 0.01; ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Morusin inhibits OM‐induced osteogenic differentiation of VICs. A) Immunoblot analysis of Runx2 and ALP expression in VICs from indicated groups (n = 3, each group). B) Bar plot showing the fold change of Runx2 expression over control. C) Bar plot showing the fold change of ALP expression over control. D) Immunofluorescent staining of ALP (green), Runx2 (red), and DAPI (blue) in the VICs from indicated groups. Scale bar 50 µm. E,F) With OM induction for 7 days, representative ALP staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. G–I) With OM induction for 21 days, representative Alizarin red staining showed the calcific nodules in VICs from indicated groups (n = 3, each group). Scale bar 50 µm. J) With OM induction for 21 days, representative Von Kossa and Alizarin Red staining of aortic valve leaflets. K) Bar plot showing the percentage of Von Kossa positive staining area of indicated groups (n = 3, each group). L) Bar plot showing the percentage of Alizarin Red positive staining area of indicated groups (n = 3, each group). Data are mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Morusin attenuates VIC calcification depending on Trim25. A,B) VICs were transfected with CCND1 siRNA or scrambled siRNA, immunoblot analysis of Trim25 expression in VICs from indicated groups (n = 3, each group). Bar plots showing the semiquantitative analysis of Trim25 expression. C) VICs were transfected with Trim25 siRNA or scrambled siRNA. Co‐IP analysis of the Keap1 ubiquitination level in VICs from indicated groups (n = 3, each group). D–M) Representative immunoblot images and quantification of the levels of Trim25, ALP, Runx2, and P21 in VICs from indicated groups (n = 3, each group). N,S) VICs were transfected with Trim25 siRNA or scrambled siRNA, representative ALP staining of VICs from indicated groups (n = 3, each group). P,U) VICs were transfected with Trim25 siRNA or scrambled siRNA, representative Alizarin red staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. Data are means ± SD. NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Analyses of TNAP expression and activity by immunofluorescence staining, Western blot, and enzyme activity assay. Cell analyses were performed in five different ALPLtg PDL-hTERT cell lines, the wt line and control PDL-hTERT that did not undergo genome editing. (A) TNAP expression (green) was detected by immunofluorescence after nuclear counterstaining with DAPI (blue). Representative images are shown. Scale bars = 50 µm. (B) Quantification of TNAP signals in IF images were done by corrected total cell fluorescence (CTCF) for ten random cells per image, and five images per cell line (n = 50). (C) Specific TNAP activity was measured by a CSPD assay confirming the spontaneous activity of the expressed protein. N = 4 per condition. (D–F) TNAP expression level and the expression of the mitochondrial protein TOMM20 in total cell lysates were determined semi-quantitatively by Western blot analysis in relation to the housekeeping protein beta -actin/ACTB. A lysate of a TNAP overexpressing cell line (ALPL over) was used as positive control. A representative immunoblot is shown in (C). White pixels indicate signal oversaturation. Quantification results in E and F are presented as mean ± SEM, N = 3 per condition. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40530336), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB29092U-100UG
Alexa Fluor 405	FAB29092V-100UG
Alexa Fluor 488	FAB29092G-100UG
Alexa Fluor 532	FAB29092X-100UG
Alexa Fluor 594	FAB29092T-100UG
Alexa Fluor 647	FAB29092R-100UG
Alexa Fluor 700	FAB29092N-100UG
Alexa Fluor 750	FAB29092S-100UG
Alexa Fluor Plus 405	FAB29092AFP405-100UG
Alexa Fluor Plus 488	FAB29092AFP488-100UG
Alexa Fluor Plus 555	FAB29092AFP555-100UG
Alexa Fluor Plus 594	FAB29092AFP594-100UG
Alexa Fluor Plus 647	FAB29092AFP647-100UG
Alexa Fluor Plus 680	FAB29092AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB29092-SP

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NBP3-15273

Alkaline Phosphatase/ALPL Antibody (10B1M5)

100 ul

NBP3-15273

Immunohistochemistry: Alkaline Phosphatase/ALPL Antibody (10B1M5) [Alkaline Phosphatase/ALPL] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Alkaline Phosphatase/ALPL Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273] -Analysis of various lysates using Alkaline Phosphatase (ALPL) Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB29091

Alkaline Phosphatase/ALPL Antibody (388828)

(6 Publications)

100 μg

MAB29091

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB29091U-100UG
Alexa Fluor 405	FAB29091V-100UG
Alexa Fluor 488	FAB29091G-100UG
Alexa Fluor 532	FAB29091X-100UG
Alexa Fluor 594	FAB29091T-100UG
Alexa Fluor 647	FAB29091R-100UG
Alexa Fluor 700	FAB29091N-100UG
Alexa Fluor 750	FAB29091S-100UG
Alexa Fluor Plus 405	FAB29091AFP405-100UG
Alexa Fluor Plus 488	FAB29091AFP488-100UG
Alexa Fluor Plus 555	FAB29091AFP555-100UG
Alexa Fluor Plus 594	FAB29091AFP594-100UG
Alexa Fluor Plus 647	FAB29091AFP647-100UG
Alexa Fluor Plus 680	FAB29091AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB29091-SP

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MAB2909

Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated]

(5 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2909U-100UG
Alexa Fluor 405	FAB2909V-100UG
Alexa Fluor 488	FAB2909G-100UG
Alexa Fluor 532	FAB2909X-100UG
Alexa Fluor 594	FAB2909T-100UG
Alexa Fluor 647	FAB2909R-100UG
Alexa Fluor 700	FAB2909N-100UG
Alexa Fluor 750	FAB2909S-100UG
Alexa Fluor Plus 405	FAB2909AFP405-100UG
Alexa Fluor Plus 488	FAB2909AFP488-100UG
Alexa Fluor Plus 555	FAB2909AFP555-100UG
Alexa Fluor Plus 594	FAB2909AFP594-100UG
Alexa Fluor Plus 647	FAB2909AFP647-100UG
Alexa Fluor Plus 680	FAB2909AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2909-SP

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AF2909

Alkaline Phosphatase/ALPL Antibody [Unconjugated]

(1 Publication)

100 μg

AF2909

HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera-2 human testicular embryonic carcinoma cell line, and mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, NTera-2 human testicular embryonic carcinoma cell line, and mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2909U-100UG
Alexa Fluor 405	AF2909V-100UG
Alexa Fluor 488	AF2909G-100UG
Alexa Fluor 532	AF2909X-100UG
Alexa Fluor 594	AF2909T-100UG
Alexa Fluor 647	AF2909R-100UG
Alexa Fluor 700	AF2909N-100UG
Alexa Fluor 750	AF2909S-100UG
Alexa Fluor Plus 405	AF2909AFP405-100UG
Alexa Fluor Plus 488	AF2909AFP488-100UG
Alexa Fluor Plus 555	AF2909AFP555-100UG
Alexa Fluor Plus 594	AF2909AFP594-100UG
Alexa Fluor Plus 647	AF2909AFP647-100UG
Alexa Fluor Plus 680	AF2909AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2909-SP

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NBP2-89225

Alkaline Phosphatase/ALPL Antibody (077) - Azide and BSA Free

100 ul

NBP2-89225

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89225AF350
Alexa Fluor 405	NBP2-89225AF405
Alexa Fluor 488	NBP2-89225AF488
Alexa Fluor 532	NBP2-89225AF532
Alexa Fluor 594	NBP2-89225AF594
Alexa Fluor 647	NBP2-89225AF647
Alexa Fluor 700	NBP2-89225AF700
Alexa Fluor 750	NBP2-89225AF750
Alexa Fluor Plus 405	NBP2-89225AFP405
Alexa Fluor Plus 488	NBP2-89225AFP488
Alexa Fluor Plus 555	NBP2-89225AFP555
Alexa Fluor Plus 594	NBP2-89225AFP594
Alexa Fluor Plus 647	NBP2-89225AFP647
Alexa Fluor Plus 680	NBP2-89225AFP680
Allophycocyanin	NBP2-89225APC
Biotin	NBP2-89225B
CoraFluor 1	NBP2-89225CL1
DyLight 350	NBP2-89225UV
DyLight 405	NBP2-89225V
DyLight 488	NBP2-89225G
DyLight 550	NBP2-89225R
DyLight 594	NBP2-89225DL594
DyLight 650	NBP2-89225C
DyLight 680	NBP2-89225FR
DyLight 755	NBP2-89225IR
FITC	NBP2-89225F
HRP	NBP2-89225H
Janelia Fluor 525	NBP2-89225JF525
Janelia Fluor 549	NBP2-89225JF549
Janelia Fluor 646	NBP2-89225JF646
Janelia Fluor 669	NBP2-89225JF669
PE	NBP2-89225PE
PerCP	NBP2-89225PCP
mFluor Violet 450 SE	NBP2-89225MFV450
mFluor Violet 500 SE	NBP2-89225MFV500
mFluor Violet 610 SE	NBP2-89225MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89225-50ul

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NBP2-89224

Alkaline Phosphatase/ALPL Antibody (071) - Azide and BSA Free

100 ul

NBP2-89224

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89224AF350
Alexa Fluor 405	NBP2-89224AF405
Alexa Fluor 488	NBP2-89224AF488
Alexa Fluor 532	NBP2-89224AF532
Alexa Fluor 594	NBP2-89224AF594
Alexa Fluor 647	NBP2-89224AF647
Alexa Fluor 700	NBP2-89224AF700
Alexa Fluor 750	NBP2-89224AF750
Alexa Fluor Plus 405	NBP2-89224AFP405
Alexa Fluor Plus 488	NBP2-89224AFP488
Alexa Fluor Plus 555	NBP2-89224AFP555
Alexa Fluor Plus 594	NBP2-89224AFP594
Alexa Fluor Plus 647	NBP2-89224AFP647
Alexa Fluor Plus 680	NBP2-89224AFP680
Allophycocyanin	NBP2-89224APC
Biotin	NBP2-89224B
CoraFluor 1	NBP2-89224CL1
DyLight 350	NBP2-89224UV
DyLight 405	NBP2-89224V
DyLight 488	NBP2-89224G
DyLight 550	NBP2-89224R
DyLight 594	NBP2-89224DL594
DyLight 650	NBP2-89224C
DyLight 680	NBP2-89224FR
DyLight 755	NBP2-89224IR
FITC	NBP2-89224F
HRP	NBP2-89224H
Janelia Fluor 525	NBP2-89224JF525
Janelia Fluor 549	NBP2-89224JF549
Janelia Fluor 646	NBP2-89224JF646
Janelia Fluor 669	NBP2-89224JF669
PE	NBP2-89224PE
PerCP	NBP2-89224PCP
mFluor Violet 450 SE	NBP2-89224MFV450
mFluor Violet 500 SE	NBP2-89224MFV500
mFluor Violet 610 SE	NBP2-89224MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89224-50ul

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NBP2-89223

Alkaline Phosphatase/ALPL Antibody (034) - Azide and BSA Free

100 ul

NBP2-89223

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89223AF350
Alexa Fluor 405	NBP2-89223AF405
Alexa Fluor 488	NBP2-89223AF488
Alexa Fluor 532	NBP2-89223AF532
Alexa Fluor 594	NBP2-89223AF594
Alexa Fluor 647	NBP2-89223AF647
Alexa Fluor 700	NBP2-89223AF700
Alexa Fluor 750	NBP2-89223AF750
Alexa Fluor Plus 488	NBP2-89223AFP488
Alexa Fluor Plus 555	NBP2-89223AFP555
Alexa Fluor Plus 594	NBP2-89223AFP594
Alexa Fluor Plus 647	NBP2-89223AFP647
Alexa Fluor Plus 680	NBP2-89223AFP680
Allophycocyanin	NBP2-89223APC
Biotin	NBP2-89223B
CoraFluor 1	NBP2-89223CL1
DyLight 350	NBP2-89223UV
DyLight 405	NBP2-89223V
DyLight 488	NBP2-89223G
DyLight 550	NBP2-89223R
DyLight 594	NBP2-89223DL594
DyLight 650	NBP2-89223C
DyLight 680	NBP2-89223FR
DyLight 755	NBP2-89223IR
FITC	NBP2-89223F
HRP	NBP2-89223H
Janelia Fluor 525	NBP2-89223JF525
Janelia Fluor 549	NBP2-89223JF549
Janelia Fluor 646	NBP2-89223JF646
Janelia Fluor 669	NBP2-89223JF669
PE	NBP2-89223PE
PerCP	NBP2-89223PCP
mFluor Violet 450 SE	NBP2-89223MFV450
mFluor Violet 500 SE	NBP2-89223MFV500
mFluor Violet 610 SE	NBP2-89223MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89223-50ul

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NBP3-51745

Alkaline Phosphatase/ALPL Antibody (24GB5855)

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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AF2909AFP647

Alkaline Phosphatase/ALPL Antibody [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 647

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

Alkaline Phosphatase/ALPL Antibodies

Alkaline Phosphatase/ALPL Antibody [Unconjugated]

Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

Alkaline Phosphatase/ALPL Antibody (928929) [Unconjugated]

Alkaline Phosphatase/ALPL Antibody (10B1M5)

Alkaline Phosphatase/ALPL Antibody (388828)

Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated]

Alkaline Phosphatase/ALPL Antibody [Unconjugated]

Alkaline Phosphatase/ALPL Antibody (077) - Azide and BSA Free

Alkaline Phosphatase/ALPL Antibody (071) - Azide and BSA Free

Alkaline Phosphatase/ALPL Antibody (034) - Azide and BSA Free

Alkaline Phosphatase/ALPL Antibody (24GB5855)

Alkaline Phosphatase/ALPL Antibody [Alexa Fluor™ Plus 647]