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Aldolase C Antibody (4A9) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-25145AF405
Alexa Fluor 488
NBP2-25145AF488
Alexa Fluor 647
NBP2-25145AF647
Alexa Fluor 700
NBP2-25145AF700
Biotin
NBP2-25145B
DyLight 350
NBP2-25145UV
DyLight 405
NBP2-25145V
DyLight 488
NBP2-25145G
DyLight 550
NBP2-25145R
DyLight 650
NBP2-25145C
DyLight 680
NBP2-25145FR
DyLight 755
NBP2-25145IR
FITC
NBP2-25145F
HRP
NBP2-25145H
Janelia Fluor 646
NBP2-25145JF646
Unconjugated
NBP2-25145
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Aldolase C Antibody (4A9) [Janelia Fluor® 549] Summary

Immunogen
The N-terminal 20 amino acids of human Aldolase C (MPHSYPALSAEQKKELSDIA). [UniProt# P09972]
Localization
Gray matter astrocytes and cells of the pia mater
Marker
pan-Astrocyte Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ALDOC
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Aldolase C Antibody (4A9) [Janelia Fluor® 549]

  • ALDC
  • aldolase 3
  • aldolase C, fructose-bisphosphate
  • Brain-type aldolase
  • EC 4.1.2.13
  • fructoaldolase C
  • fructose-1,6-biphosphate triosephosphate lyase
  • fructose-bisphosphate aldolase C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.
Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.
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(326 Publications)
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EAAT4 Antibody
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Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48740] - Staining of human cell line A-431 shows localization to intermediate filaments.
Immunohistochemistry-Paraffin: EAAT4 Antibody [NBP2-48740] - Staining in human cerebral cortex and kidney tissues using anti-SLC1A6 antibody. Corresponding SLC1A6 RNA-seq data are presented for the same tissues.
Immunocytochemistry/Immunofluorescence: EAAT4 Antibody [NBP2-48740] - Staining of human cell line A-431 shows localization to intermediate filaments.
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GFAP Antibody
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Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).
Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.
(12 Reviews)
(59 Publications)
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Triosephosphate
isomerase Antibody
NBP1-31470
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat and human cells. Immunodetection of Eno1, Tpi1, Pgam1 and beta2-Tub (load control) in Poly I:C transfected and Control PaKiT03 cells at 4, 8 and 20 hpt. Image collected and cropped by CiteAb from the following publication (http://www.proteomesci.com/content/13/1/25), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase stained by Triosephosphate isomerase antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.
(1 Review)
(8 Publications)
Species: Hu, Mu, Rt, Bv, V-Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
GALK1 Antibody
NBP1-81853
Western Blot: GALK1 Antibody [NBP1-81853] - Analysis using Anti-GALK1 antibody NBP1-81853 (A) shows similar pattern to independent antibody NBP1-81854 (B).
Immunocytochemistry/Immunofluorescence: GALK1 Antibody [NBP1-81853] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: GALK1 Antibody [NBP1-81853] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Aldolase B Antibody
NBP2-15344
Western Blot: Aldolase B Antibody [NBP2-15344] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Aldolase B Antibody [NBP2-15344] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry: Aldolase B Antibody [NBP2-15344] - Hepatoma tissue; antibody dilution 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PGK1 Antibody
NBP2-19784
Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.
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(1 Publication)
Species: Hu, Mu, Rt, Ye
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA
PGPEP-1 Antibody
AF6278
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Hexokinase 1
Antibody (ST47-05)
NBP2-67503
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Immunocytochemistry/Immunofluorescence: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Staining Hexokinase 1 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Hexokinase 1 Antibody (ST47-05) [NBP2-67503] - Analysis of paraffin-embedded mouse skin tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin.
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(1 Publication)
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NB300-223
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Peroxiredoxin 6
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H00009588-M01
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Western Blot: Peroxiredoxin 6 Antibody (3A10-2A11) [H00009588-M01] - PRDX6 monoclonal antibody (M01), clone 3A10-2A11. Analysis of PRDX6 expression in human liver.
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Enolase 1 Antibody
(8G8)
H00002023-M01
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Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
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Doublecortin
Antibody (3E1)
NBP1-92684
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Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.
Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.
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Glutamate
Dehydrogenase Antibod
NBP2-16679
Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody [NBP2-16679] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP2-16679] - Paraffin-embedded rat brain. Glutamate Dehydrogenase antibody diluted at 1:750.
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AK5 Antibody
(OTI2F9)
NBP2-02978
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Immunocytochemistry/Immunofluorescence: AK5 Antibody (2F9) [NBP2-02978] Staining of COS7 cells transiently transfected by pCMV6-ENTRY AK5.
Immunohistochemistry-Paraffin: AK5 Antibody (2F9) [NBP2-02978] - Staining of paraffin-embedded Human prostate tissue using anti-AK5 mouse monoclonal antibody.
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ACADS Antibody
NBP1-89290
Western Blot: ACADS Antibody [NBP1-89290] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: ACADS Antibody [NBP1-89290] - Staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACADS Antibody [NBP1-89290] - Analysis in human duodenum and tonsil tissues. Corresponding ACADS RNA-seq data are presented for the same tissues.
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Transketolase
Antibody
NBP1-87441
Immunohistochemistry-Paraffin: Transketolase Antibody [NBP1-87441] - Staining in human bone marrow and skeletal muscle tissues using anti-TKT antibody. Corresponding TKT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Transketolase Antibody [NBP1-87441] - Staining of human bone marrow, cerebral cortex, skeletal muscle and testis using Anti-TKT antibody NBP1-87441 (A) shows similar protein distribution across tissues to independent antibody NBP1-87442 (B).
Western Blot: Transketolase Antibody [NBP1-87441] - Western blot analysis in human cell lines A-549 and U2OS. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
(1 Review)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(20 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
ACAT1 Antibody
NBP1-89284
Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).
Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.
(1 Review)
(5 Publications)
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P, KD
Thymidine Kinase 1
Antibody
NBP2-20626
Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - Wild-type (WT) and Thymidine Kinase 1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody [NBP2-20626] - Thymidine Kinase 1 antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Thymidine Kinase 1 stained by Thymidine Kinase 1 antibody diluted at 1:500.
Western Blot: Thymidine Kinase 1 Antibody [NBP2-20626] - A431 whole cell and cytoplasm extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Thymidine Kinase 1 antibody diluted at 1:1000.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-25145AF405
Alexa Fluor 488 NBP2-25145AF488
Alexa Fluor 647 NBP2-25145AF647
Alexa Fluor 700 NBP2-25145AF700
Biotin NBP2-25145B
DyLight 350 NBP2-25145UV
DyLight 405 NBP2-25145V
DyLight 488 NBP2-25145G
DyLight 550 NBP2-25145R
DyLight 650 NBP2-25145C
DyLight 680 NBP2-25145FR
DyLight 755 NBP2-25145IR
FITC NBP2-25145F
HRP NBP2-25145H
Janelia Fluor 549 NBP2-25145JF549
Janelia Fluor 646 NBP2-25145JF646
Learn about Custom Antibody Labeling

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RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism
By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,...  Read full blog post.
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Bioinformatics

Gene Symbol ALDOC