Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody - BSA Free

Western Blot: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Whole cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Frozen: Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] - Analysis of frozen bovine cornea section, using ALDH3A1 antibody at 1:100 dilution.

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody [NBP2-15340] -Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH3A1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Mu, Bv, Ca, Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Aldehyde Dehydrogenase 3-A1/ALDH3A1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH3A1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody - BSA Free

• aldehyde dehydrogenase 3 family, member A1
• Aldehyde dehydrogenase 3
• Aldehyde Dehydrogenase 3A1
• Aldehyde Dehydrogenase 3-A1
• Aldehyde dehydrogenase family 3 member A1
• aldehyde dehydrogenase isozyme 3
• aldehyde dehydrogenase type III
• ALDH3A1
• ALDH3aldehyde dehydrogenase, dimeric NADP-preferring
• ALDHIII
• EC 1.2.1
• EC 1.2.1.5
• MGC10406
• stomach aldehyde dehydrogenase

Background

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Aldehyde Dehydrogenase 3-A1/ALDH3A1 Antibody (NBP2-15340)(1)

Publication using NBP2-15340

• Weiss-Sadan T, Ge M, Hayashi M et al. NRF2 activation induces NADH-reductive stress, providing a metabolic vulnerability in lung cancer Cell metabolism 2023-02-22 [PMID: 36841242] (ICC/IF)

Bioinformatics

• Gene Symbol: ALDH3A1
• Uniprot:
  • P30838()