Aldehyde dehydrogenase 5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ALDH1B1 protein stained by ALDH1B1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Paraffin-embedded mouse duodenum. ALDH1B1 antibody diluted at 1:500.

Immunohistochemistry-Paraffin: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:500 dilution.

Western Blot: Aldehyde dehydrogenase 5 Antibody [NBP1-32569] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aldehyde dehydrogenase 5 antibody (NBP1-32569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aldehyde dehydrogenase 5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Aldehyde dehydrogenase 5 Antibody - BSA Free (NBP1-32569) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Aldehyde dehydrogenase 5. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Predicted Species: Rat (94%), Primate (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALDH1B1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (99%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Aldehyde dehydrogenase 5 Antibody - BSA Free

• aldehyde dehydrogenase 1 family, member B1
• Aldehyde dehydrogenase 5
• Aldehyde dehydrogenase family 1 member B1
• ALDH class 2
• ALDH5aldehyde dehydrogenase X, mitochondrial
• ALDHXacetaldehyde dehydrogenase 5
• EC 1.2.1
• EC 1.2.1.3
• MGC2230
• mitochondrial aldehyde dehydrogenase X

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. This gene does not contain introns in the coding sequence. The variation of this locus may affect the development of alcohol-related problems. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ALDH1B1
• Entrez:
  • 219(Human)
• Uniprot:
  • P30837(Human)