Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G)

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G) [NBP2-80139] - Detection of human ALDH1A1 by WB of HeLa, HEK293T, MCF-7, Hep-G2, Jurkat, SK-MEL-28, GaMG and A-549 lysate. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal [BLR089G] (NBP2-80139). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal.

Immunocytochemistry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G) [NBP2-80139] - Detection of human ALDH1A1 in FFPE NCI-H2286 cells by ICC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal [BLR089G] (NBP2-80139). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G) [NBP2-80139] - Detection of human ALDH1A1 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal [BLR089G] (NBP2-80139). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G) [NBP2-80139] - Detection of human ALDH1A1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-ALDH1A1 recombinant monoclonal [BLR089G] (NBP2-80139). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G) [NBP2-80139] - Detection of human ALDH1A1 by WB of immunoprecipitates from A-549 lysate. Antibodies: Rabbit anti-ALDH1A1 recombinant monoclonal [BLR089G] (NBP2-80139) and another rabbit anti-ALDH1A1 recombinant monoclonal.

Product Discontinued

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: between 300 and 350
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ALDH1A1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100 - 1:500
  • Immunoprecipitation 12 ul / 1 mg lysate
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Borate Buffered Saline (BBS) pH 8.2, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (BLR089G)

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A1