Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot Analysis of human Liver tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot of K562 and Lung lysate Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: ALDH1A1/1381
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54422)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 315-434) of human Aldehyde Dehydrogenase 1-A1/ALDH1A1 protein (exact sequence is proprietary). (Uniprot: P00352)
• Localization: Cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381)

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A1
• Uniprot:
  • P00352()
  • P00352()