Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (5A11)

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (5A11) [NBP1-51654] - Western blot analysis using ALDH1A1 mouse mAb against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (5A11) [NBP1-51654] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and lung cancer (right) using ALDH1A1 mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 5A11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (5A11) Summary

• Immunogen: Purified recombinant fragment of human ALDH1A1 expressed in E. Coli.
• Marker: Astrocyte Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH1A1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (5A11)

• acetaldehyde dehydrogenase 1
• ALDC
• aldehyde dehydrogenase 1 family, member A1
• aldehyde dehydrogenase 1, soluble
• Aldehyde Dehydrogenase 1A1
• Aldehyde Dehydrogenase 1-A1
• Aldehyde dehydrogenase family 1 member A1
• Aldehyde dehydrogenase, cytosolic
• aldehyde dehydrogenase, liver cytosolic
• ALDH class 1
• ALDH1
• ALDH11
• ALDH1A1
• ALDH-E1
• ALHDII
• EC 1.2.1
• EC 1.2.1.36
• MGC2318
• PUMB1
• RALDH 1
• RALDH1
• retinal dehydrogenase 1
• retinaldehyde dehydrogenase 1

Background

Usual human livers contain two major ALDH isozymes, i.e., cytosolic ALDH1 and mitochondrial ALDH2(1). Human red cell aldehyde dehydrogenase (ALDH) resembles the liver cytosolic isozyme in numerous physicochemical properties. Thus, the erythrocyte enzyme, by virtue of its chemical and structural identity with the liver cytosolic enzyme, may serve as a suitable peripheral enzyme model to understand the cause and mechanism of alcohol abuse-related changes in liver cytosolic ALDH that has been found to be reduced in alcoholics (2). ALDH1A1 is known to catalyze the oxidation of retinaldehyde to retinoic acid. Overexpression of human ALDH1A1 in HEK cells led to a more than 20-fold increase in 3-deoxyglucosone dehydrogenase activity (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH1A1