alcohol dehydrogenase 5 Antibody - BSA Free

Western Blot: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Western blot analysis of various lysates using alcohol dehydrogenase 5 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of NIH/3T3 cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of PC-12 cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of HepG2 cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: alcohol dehydrogenase 5 Antibody [NBP3-35343] - Immunofluorescence analysis of HeLa cells using alcohol dehydrogenase 5 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alcohol dehydrogenase 5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit alcohol dehydrogenase 5 Antibody - BSA Free (NBP3-35343) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human alcohol dehydrogenase 5 (NP_000662.3).
  
  Sequence:
  MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADH5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:100 - 1:500
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for alcohol dehydrogenase 5 Antibody - BSA Free

• ADH-3
• ADHXEC 1.1.1.1
• alcohol dehydrogenase (class III), chi polypeptide
• alcohol dehydrogenase 5 (class III), chi polypeptide
• Alcohol dehydrogenase 5
• Alcohol dehydrogenase class chi chain
• alcohol dehydrogenase class-3
• Alcohol dehydrogenase class-III
• EC 1.1.1
• EC 1.1.1.-
• EC 1.1.1.284
• FALDH
• FDHGSNOR
• formaldehyde dehydrogenase
• Glutathione-dependent formaldehyde dehydrogenase
• GSH-FDH
• S-(hydroxymethyl)glutathione dehydrogenase

Background

The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADH5