Rat Albumin ELISA Kit (Colorimetric) Summary
Specificity |
This assay has high sensitivity and excellent specificity for detection of rat MAU. No significant cross-reactivity or interference between rat MAU and analogues was observed. |
Standard Curve Range |
0.01 ug/ml-40 ug/ml |
Sensitivity |
0.01 ug/ml |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 10% |
Intra-Assay |
CV% < 8% |
Sample Volume |
50-100ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
ALB |
Packaging, Storage & Formulations
Kit Components
Components
|
- Sample Diluent
- Standard (100 x concentrate)
- Stop Solution
- Wash Buffer (25 x concentrate)
- TMB Substrate
- Adhesive Strip (For 96 wells)
- Assay plate
- Biotin-antibody Diluent
- Biotin-antibody (100 x concentrate)
- Instruction manual
- HRP-avidin Diluent
- HRP-avidin (100 x concentrate)
|
Notes
Please contact our Technical Services with any questions regarding species reactivity
Alternate Names for Rat Albumin ELISA Kit (Colorimetric)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAU bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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